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SEC13 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - SEC13 Antibody (Center) AP10738c
    SEC13 Antibody (Center) (Cat. #AP10738c) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the SEC13 antibody detected the SEC13 protein (arrow).
  • FC - SEC13 Antibody (Center) AP10738c
    SEC13 Antibody (Center) (Cat. #AP10738c) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P55735
Other Accession NP_899195.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 35541 Da
Antigen Region 72-100 aa
Additional Information
Gene ID 6396
Other Names Protein SEC13 homolog, SEC13-like protein 1, SEC13-related protein, SEC13, D3S1231E, SEC13L1, SEC13R
Target/Specificity This SEC13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 72-100 amino acids from the Central region of human SEC13.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSEC13 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SEC13
Synonyms D3S1231E, SEC13L1, SEC13R
Function Functions as a component of the nuclear pore complex (NPC) and the COPII coat. At the endoplasmic reticulum, SEC13 is involved in the biogenesis of COPII-coated vesicles. As a component of the GATOR2 complex, inhibits GATOR1 complex, an inhibitor of the amino acid-sensing branch of the TORC1 pathway.
Cellular Location Cytoplasmic vesicle, COPII-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus, nuclear pore complex. Note=In interphase, localizes at both sides of the NPC
Research Areas
Citations (0)

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The protein encoded by this gene belongs to the SEC13 family of WD-repeat proteins. It is a constituent of the endoplasmic reticulum and the nuclear pore complex. It has similarity to the yeast SEC13 protein, which is required for vesicle biogenesis from endoplasmic reticulum during the transport of proteins. Multiple alternatively spliced transcript variants have been found.


Nielsen, A.L. Biochem. Biophys. Res. Commun. 388(3):571-575(2009)
Townley, A.K., et al. J. Cell. Sci. 121 (PT 18), 3025-3034 (2008) :
Stagg, S.M., et al. Cell 134(3):474-484(2008)
Hsia, K.C., et al. Cell 131(7):1313-1326(2007)
Glavy, J.S., et al. Proc. Natl. Acad. Sci. U.S.A. 104(10):3811-3816(2007)

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Cat# AP10738c
(40 western blots)
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