TMEM66 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, FC, E |
---|---|
Primary Accession | Q96BY9 |
Other Accession | NP_057211.4 |
Reactivity | Human, Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 36975 Da |
Antigen Region | 33-62 aa |
Gene ID | 51669 |
---|---|
Other Names | Store-operated calcium entry-associated regulatory factor, SARAF, SOCE-associated regulatory factor, HBV X-transactivated gene 3 protein, HBV XAg-transactivated protein 3, Protein FOAP-7, Transmembrane protein 66, SARAF, TMEM66, XTP3 |
Target/Specificity | This TMEM66 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33-62 amino acids from the N-terminal region of human TMEM66. |
Dilution | WB~~1:1000 FC~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | TMEM66 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | SARAF |
---|---|
Synonyms | TMEM66, XTP3 |
Function | Negative regulator of store-operated Ca(2+) entry (SOCE) involved in protecting cells from Ca(2+) overfilling. In response to cytosolic Ca(2+) elevation after endoplasmic reticulum Ca(2+) refilling, promotes a slow inactivation of STIM (STIM1 or STIM2)- dependent SOCE activity: possibly act by facilitating the deoligomerization of STIM to efficiently turn off ORAI when the endoplasmic reticulum lumen is filled with the appropriate Ca(2+) levels, and thus preventing the overload of the cell with excessive Ca(2+) ions. |
Cellular Location | Endoplasmic reticulum membrane; Single-pass type I membrane protein. Note=Translocates to the endoplasmic reticulum-plasma membrane (ER-PM) region in a STIM1-dependent manner following cytosolic Ca(2+) elevation |
Tissue Location | Highly expressed in macrophages. {ECO:0000269|Ref.2} |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
TMEM66 is a multi-pass membrane protein which belongs to the TMEM66 family. The exact function of TMEM66 remains unknown.
References
Rose, J. Phd, et al. Mol. Med. (2010) In press :
Zhang, Z., et al. Protein Sci. 13(10):2819-2824(2004)
Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003)
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.