|Application ||WB, E|
|Calculated MW||141466 Da|
|Antigen Region||979-1010 aa|
|Other Names||Histone-lysine N-methyltransferase EHMT1, 211-, Euchromatic histone-lysine N-methyltransferase 1, Eu-HMTase1, G9a-like protein 1, GLP, GLP1, Histone H3-K9 methyltransferase 5, H3-K9-HMTase 5, Lysine N-methyltransferase 1D, EHMT1, EUHMTASE1, GLP, KIAA1876, KMT1D|
|Target/Specificity||This EHMT1 (EUHMTASE1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 979-1010 amino acids from the Central region of human EHMT1 (EUHMTASE1).|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||EHMT1 (EUHMTASE1) Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||EUHMTASE1, GLP, KIAA1876, KMT1D|
|Function||Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in G0/G1 transition in cell cycle. In addition to the histone methyltransferase activity, also methylates non- histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53.|
|Cellular Location||Nucleus. Chromosome. Note=Associates with euchromatic regions|
|Tissue Location||Widely expressed.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
EHMT1, also known as EUHMTASE1, is a histone methyltransferase. This protein methylates 'Lys-9' of histone H3 in vitro. H3 'Lys-9' methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. EHMT1 is Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in the G0/G1 transition of the cell cycle.
Ogawa, H., et al., Science 296(5570):1132-1136 (2002).
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.