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TJAP1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - TJAP1 Antibody (N-term) AP10762a
    TJAP1 Antibody (N-term) (Cat. #AP10762a) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the TJAP1 antibody detected the TJAP1 protein (arrow).
  • IHC-P - TJAP1 Antibody (N-term) AP10762a
    TJAP1 antibody (N-term) (Cat. #AP10762a) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TJAP1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q5JTD0
Other Accession Q9DCD5, NP_001139488.1
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Antigen Region 144-171 aa
Additional Information
Other Names Tight junction-associated protein 1, Protein incorporated later into tight junctions, Tight junction protein 4, TJAP1, PILT, TJP4
Target/Specificity This TJAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 144-171 amino acids from the N-terminal region of human TJAP1.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTJAP1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TJAP1
Synonyms PILT, TJP4
Cellular Location Golgi apparatus. Cell junction, tight junction. Note=Recruited to tight junctions (TJ) during late stages of maturation of the TJ complexes. Excluded from adherens junctions and desmosomes (By similarity).
Tissue Location Ubiquitously expressed. EMBL; AK024269; BAB14867.1; -; mRNA EMBL; CR749480; CAH18308.1; -; mRNA EMBL; AL117398; CAI46221.1; -; Transcribed_RNA EMBL; AL355802; CAI42617.1; -; Genomic_DNA EMBL; AL355802; CAI42618.1; -; Genomic_DNA EMBL; AL355802; CAI42619.1; -; Genomic_DNA EMBL; BC046239; AAH46239.1; -; mRNA EMBL; BC064401; AAH64401.1; -; mRNA CCDS; CCDS4898.1; -. [Q5JTD0-2] CCDS; CCDS55004.1; -. [Q5JTD0-1] RefSeq; NP_001139488.1; NM_001146016.1. [Q5JTD0-1] RefSeq; NP_001139489.1; NM_001146017.1. [Q5JTD0-1] RefSeq; NP_001139490.1; NM_001146018.1. [Q5JTD0-2] RefSeq; NP_001139491.1; NM_001146019.1. [Q5JTD0-2] RefSeq; NP_001139492.1; NM_001146020.1. [Q5JTD0-2] RefSeq; NP_542171.2; NM_080604.2. [Q5JTD0-2] RefSeq; XP_006715312.1; XM_006715249.1. [Q5JTD0-1] RefSeq; XP_006715313.1; XM_006715250.3. [Q5JTD0-1] RefSeq; XP_006715314.1; XM_006715251.2. [Q5JTD0-1] RefSeq; XP_006715315.1; XM_006715252.2. [Q5JTD0-1] RefSeq; XP_006715317.1; XM_006715254.3. [Q5JTD0-1] RefSeq; XP_006715319.1; XM_006715256.1. [Q5JTD0-1] RefSeq; XP_006715320.1; XM_006715257.2. [Q5JTD0-1] RefSeq; XP_006715322.1; XM_006715259.3. [Q5JTD0-1] RefSeq; XP_006715323.1; XM_006715260.1. [Q5JTD0-1] RefSeq; XP_006715324.1; XM_006715261.1. [Q5JTD0-1] RefSeq; XP_006715325.1; XM_006715262.2. [Q5JTD0-1] RefSeq; XP_006715326.1; XM_006715263.2. [Q5JTD0-1] RefSeq; XP_006715328.1; XM_006715265.1. [Q5JTD0-2] RefSeq; XP_006715329.1; XM_006715266.1. [Q5JTD0-2] RefSeq; XP_011513297.1; XM_011514995.1. [Q5JTD0-1] RefSeq; XP_016866977.1; XM_017011488.1. [Q5JTD0-1] RefSeq; XP_016866978.1; XM_017011489.1. [Q5JTD0-1] RefSeq; XP_016866979.1; XM_017011490.1. [Q5JTD0-2] RefSeq; XP_016866980.1; XM_017011491.1. [Q5JTD0-2] RefSeq; XP_016866981.1; XM_017011492.1. [Q5JTD0-2] RefSeq; XP_016866982.1; XM_017011493.1. [Q5JTD0-2] UniGene; Hs.520145; - ProteinModelPortal; Q5JTD0; - SMR; Q5JTD0; - BioGrid; 125043; 19 IntAct; Q5JTD0; 18 STRING; 9606.ENSP00000361522; - iPTMnet; Q5JTD0; - PhosphoSitePlus; Q5JTD0; - BioMuta; TJAP1; - DMDM; 74742134; - EPD; Q5JTD0; - MaxQB; Q5JTD0; - PaxDb; Q5JTD0; - PeptideAtlas; Q5JTD0; - PRIDE; Q5JTD0; - Ensembl; ENST00000259751; ENSP00000259751; ENSG00000137221. [Q5JTD0-2] Ensembl; ENST00000372444; ENSP00000361521; ENSG00000137221. [Q5JTD0-2] Ensembl; ENST00000372445; ENSP00000361522; ENSG00000137221. [Q5JTD0-1] Ensembl; ENST00000372449; ENSP00000361527; ENSG00000137221. [Q5JTD0-1] Ensembl; ENST00000372452; ENSP00000361530; ENSG00000137221. [Q5JTD0-2] Ensembl; ENST00000436109; ENSP00000407080; ENSG00000137221. [Q5JTD0-2] Ensembl; ENST00000438588; ENSP00000408769; ENSG00000137221. [Q5JTD0-1] Ensembl; ENST00000612912; ENSP00000484142; ENSG00000137221. [Q5JTD0-3] GeneID; 93643; - KEGG; hsa:93643; - UCSC; uc003ovc.3; human. [Q5JTD0-1] CTD; 93643; - DisGeNET; 93643; - GeneCards; TJAP1; - H-InvDB; HIX0165025; - HGNC; HGNC:17949; TJAP1 HPA; HPA030164; - HPA; HPA030165; - HPA; HPA030166; - MIM; 612658; gene neXtProt; NX_Q5JTD0; - OpenTargets; ENSG00000137221; - PharmGKB; PA38268; - eggNOG; ENOG410IJKM; Eukaryota eggNOG; ENOG410Z76C; LUCA GeneTree; ENSGT00530000063778; - HOVERGEN; HBG056279; - InParanoid; Q5JTD0; - KO; K06105; - OMA; MSEGVPG; - OrthoDB; EOG091G03KA; - PhylomeDB; Q5JTD0; - TreeFam; TF331612; - ChiTaRS; TJAP1; human GeneWiki; TJAP1; - GenomeRNAi; 93643; - PRO; PR:Q5JTD0; - Proteomes; UP000005640; Chromosome 6 Bgee; ENSG00000137221; - CleanEx; HS_TJAP1; - ExpressionAtlas; Q5JTD0; baseline and differential Genevisible; Q5JTD0; HS GO; GO:0005923; C:bicellular tight junction; IEA:UniProtKB-SubCell GO; GO:0005768; C:endosome; IEA:Ensembl GO; GO:0005886; C:plasma membrane; IEA:Ensembl GO; GO:0005802; C:trans-Golgi network; IEA:Ensembl GO; GO:0007030; P:Golgi organization; IEA:Ensembl InterPro; IPR028179; Tjap1 PANTHER; PTHR28664:SF3; PTHR28664:SF3; 1 Pfam; PF15453; Pilt; 2 1: Evidence at protein level; Acetylation; Alternative splicing; Cell junction; Coiled coil; Complete proteome; Golgi apparatus; Phosphoprotein; Reference proteome; Tight junction INIT_MET 1 1 Removed. CHAIN 2 557 Tight junction-associated protein 1 /FTId=PRO_0000072550 COILED 36 175 COMPBIAS 271 327 Pro-rich MOD_RES 2 2 N-acetylthreonine MOD_RES 300 300 Phosphoserine MOD_RES 318 318 Phosphothreonine MOD_RES 320 320 Phosphoserine MOD_RES 345 345 Phosphoserine MOD_RES 422 422 Phosphothreonine MOD_RES 491 491 Phosphoserine MOD_RES 545 545 Phosphoserine VAR_SEQ 1 65 Missing (in isoform 4) /FTId=VSP_015999 VAR_SEQ 44 557 Missing (in isoform 3) /FTId=VSP_016000 VAR_SEQ 66 66 L -> M (in isoform 4) /FTId=VSP_016001 VAR_SEQ 120 129 Missing (in isoform 2 and isoform 4) /FTId=VSP_016002 CONFLICT 174 174 L -> P (in Ref. 1; BAB14867) CONFLICT 185 186 HF -> QV (in Ref. 2; CAI46221) SEQUENCE 557 AA; 61821 MW; 07D41B72E1CB0E20 CRC64; MTSAAPAKKP YRKAPPEHRE LRLEIPGSRL EQEEPLTDAE RMKLLQEENE ELRRRLASAT RRTEALEREL EIGQDCLELE LGQSREELDK FKDKFRRLQN SYTASQRTNQ ELEDKLHTLA SLSHSWIFAI KKAEMDRKTL DWEIVELTNK LLDAKNTINK LEELNERYRL DCNLAVQLLK CNKSHFRNHK FADLPCELQD MVRKHLHSGQ EAASPGPAPS LAPGAVVPTS VIARVLEKPE SLLLNSAQSG SAGRPLAEDV FVHVDMSEGV PGDPASPPAP GSPTPQPNGE CHSLGTARGS PEEELPLPAF EKLNPYPTPS PPHPLYPGRR VIEFSEDKVR IPRNSPLPNC TYATRQAISL SLVEEGSERA RPSPVPSTPA SAQASPHHQP SPAPLTLSAP ASSASSEEDL LVSWQRAFVD RTPPPAAVAQ RTAFGRDALP ELQRHFAHSP ADRDEVVQAP SARPEESELL LPTEPDSGFP REEEELNLPI SPEEERQSLL PINRGTEEGP GTSHTEGRAW PLPSSSRPQR SPKRMGVHHL HRKDSLTQAQ EQGNLLN
Research Areas
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Background

This gene encodes a tight junction-associated protein. Incorporation of the encoded protein into tight junctions occurs at a late stage of formation of the junctions. The encoded protein localizes to the Golgi and may function in vesicle trafficking. Alternatively spliced transcript variants have been described. A related pseudogene exists on the X chromosome. [provided by RefSeq].

References

Kimura, K., et al. Genome Res. 16(1):55-65(2006)
Kawabe, H., et al. J. Biol. Chem. 276(51):48350-48355(2001)

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Cat# AP10762a
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