- CITATIONS: 1
|Application ||WB, IHC-P, FC, E|
|Other Accession||Q5U2U0, Q9JHS4, NP_006651.2|
|Calculated MW||69224 Da|
|Antigen Region||449-477 aa|
|Other Names||ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial, CLPX|
|Target/Specificity||This CLPX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 449-477 amino acids from the C-terminal region of human CLPX.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CLPX Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||ATP-dependent specificity component of the Clp protease complex. Hydrolyzes ATP. Targets specific substrates for degradation by the Clp complex (PubMed:11923310, PubMed:22710082). Can perform chaperone functions in the absence of CLPP. Enhances the DNA-binding activity of TFAM and is required for maintaining a normal mitochondrial nucleoid structure (PubMed:22841477). ATP- dependent unfoldase that stimulates the incorporation of the pyridoxal phosphate cofactor into 5-aminolevulinate synthase, thereby activating 5-aminolevulinate (ALA) synthesis, the first step in heme biosynthesis. Important for efficient erythropoiesis through upregulation of heme biosynthesis (PubMed:25957689).|
|Cellular Location||Mitochondrion. Mitochondrion matrix, mitochondrion nucleoid|
|Tissue Location||Higher expression in skeletal muscle and heart and to a lesser extent in liver, brain, placenta, lung, kidney and pancreas.|
Provided below are standard protocols that you may find useful for product applications.
ATP-dependent specificity component of the Clp protease. It directs the protease to specific substrates. Can perform chaperone functions in the absence of clpP (By similarity).
Martin, A., et al. Mol. Cell 29(4):441-450(2008) Bogenhagen, D.F., et al. J. Biol. Chem. 283(6):3665-3675(2008) Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) : Kang, S.G., et al. J. Biol. Chem. 280(42):35424-35432(2005) Kang, S.G., et al. J. Biol. Chem. 277(23):21095-21102(2002)
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