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HOMEZ Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - HOMEZ Antibody  (N-term) AP10772a
    Fluorescent confocal image of A431 cell stained with HOMEZ Antibody (N-term)(Cat#AP10772a).A431 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HOMEZ primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). HOMEZ immunoreactivity is localized to Nucleus significantly.
    detail
  • WB - HOMEZ Antibody  (N-term) AP10772a
    HOMEZ Antibody (N-term) (Cat. #AP10772a) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the HOMEZ antibody detected the HOMEZ protein (arrow).
    detail
  • FC - HOMEZ Antibody  (N-term) AP10772a
    HOMEZ Antibody (N-term) (Cat. #AP10772a) flow cytometric analysis of NIH-3T3 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IF, FC, WB, E
Primary Accession Q8IX15
Other Accession NP_065885.2
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 61240 Da
Antigen Region 158-186 aa
Additional Information
Gene ID 57594
Other Names Homeobox and leucine zipper protein Homez, Homeodomain leucine zipper-containing factor, HOMEZ, KIAA1443
Target/Specificity This HOMEZ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 158-186 amino acids from the N-terminal region of human HOMEZ.
Dilution IF~~1:10~50
WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHOMEZ Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HOMEZ
Synonyms KIAA1443
Function May function as a transcriptional regulator.
Cellular Location Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108, ECO:0000269|PubMed:12925734}
Tissue Location Ubiquitous. Strongly expressed in adult testis and kidney as well as fetal lung and kidney.
Research Areas
Citations (0)
citation

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Background

May function as a transcriptional regulator.

References

Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004)
Bayarsaihan, D., et al. Proc. Natl. Acad. Sci. U.S.A. 100(18):10358-10363(2003)
Ceulemans, H., et al. Bioessays 24(4):371-381(2002)

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$ 182.50
$ 70.00
Cat# AP10772a
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