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SACA1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - SACA1 Antibody  (N-term) AP10775A
    SACA1 Antibody (N-term) (Cat. #AP10775a) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the SACA1 antibody detected the SACA1 protein (arrow).
  • IHC-P - SACA1 Antibody  (N-term) AP10775A
    SACA1 antibody (N-term) (Cat. #AP10775a) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SACA1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - SACA1 Antibody  (N-term) AP10775A
    SACA1 Antibody (N-term) (Cat. #AP10775a) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9HBV2
Other Accession NP_112222.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Antigen Region 46-74 aa
Additional Information
Other Names Sperm acrosome membrane-associated protein 1, Sperm acrosomal membrane-associated protein 32, SPACA1, SAMP32
Target/Specificity This SACA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46-74 amino acids from the N-terminal region of human SACA1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSACA1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms SAMP32
Function May be involved in sperm-egg fusion.
Cellular Location Membrane; Single-pass type I membrane protein
Tissue Location Testis specific. At the equatorial segment and the inner acrosomal membrane of capacitated sperm EMBL; AF203447; AAG31422.1; -; mRNA EMBL; AL136096; CAI19915.1; -; Genomic_DNA EMBL; BC029488; AAH29488.1; -; mRNA CCDS; CCDS5014.1; - RefSeq; NP_112222.1; NM_030960.2 UniGene; Hs.161241; - ProteinModelPortal; Q9HBV2; - SMR; Q9HBV2; - BioGrid; 123591; 65 STRING; 9606.ENSP00000237201; - iPTMnet; Q9HBV2; - PhosphoSitePlus; Q9HBV2; - BioMuta; SPACA1; - DMDM; 74752784; - PaxDb; Q9HBV2; - PeptideAtlas; Q9HBV2; - PRIDE; Q9HBV2; - Ensembl; ENST00000237201; ENSP00000237201; ENSG00000118434 GeneID; 81833; - KEGG; hsa:81833; - UCSC; uc003pmn.3; human CTD; 81833; - DisGeNET; 81833; - GeneCards; SPACA1; - HGNC; HGNC:14967; SPACA1 HPA; HPA026744; - HPA; HPA043297; - MIM; 612739; gene neXtProt; NX_Q9HBV2; - OpenTargets; ENSG00000118434; - PharmGKB; PA37943; - eggNOG; ENOG410IFV6; Eukaryota eggNOG; ENOG410YPX9; LUCA GeneTree; ENSGT00390000004211; - HOGENOM; HOG000049221; - HOVERGEN; HBG080320; - InParanoid; Q9HBV2; - OMA; WGAKAST; - OrthoDB; EOG091G0M9J; - PhylomeDB; Q9HBV2; - TreeFam; TF336918; - BioCyc; ZFISH:ENSG00000118434-MONOMER; - GenomeRNAi; 81833; - PRO; PR:Q9HBV2; - Proteomes; UP000005640; Chromosome 6 Bgee; ENSG00000118434; - CleanEx; HS_SPACA1; - Genevisible; Q9HBV2; HS GO; GO:0002080; C:acrosomal membrane; IBA:GO_Central GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW GO; GO:0001675; P:acrosome assembly; IBA:GO_Central 1: Evidence at protein level; Complete proteome; Direct protein sequencing; Glycoprotein; Membrane; Phosphoprotein; Polymorphism; Reference proteome; Signal; Transmembrane; Transmembrane helix SIGNAL 1 29 CHAIN 30 294 Sperm acrosome membrane-associated protein 1 /FTId=PRO_0000248152 TOPO_DOM 30 221 Extracellular. TRANSMEM 222 242 Helical. TOPO_DOM 243 294 Cytoplasmic. MOD_RES 256 256 Phosphoserine MOD_RES 290 290 Phosphoserine {ECO:0000250|UniProtKB:Q9DA48} CARBOHYD 31 31 N-linked (GlcNAc...). VARIANT 237 237 L -> S (in dbSNP:rs2276089) /FTId=VAR_027258 SEQUENCE 294 AA; 32143 MW; 60DB3107E1B03D12 CRC64; MSPRGTGCSA GLLMTVGWLL LAGLQSARGT NVTAAVQDAG LAHEGEGEEE TENNDSETAE NYAPPETEDV SNRNVVKEVE FGMCTVTCGI GVREVILTNG CPGGESKCVV RVEECRGPTD CGWGKPISES LESVRLACIH TSPLNRFKYM WKLLRQDQQS IILVNDSAIL EVRKESHPLA FECDTLDNNE IVATIKFTVY TSSELQMRRS SLPATDAALI FVLTIGVIIC VFIIFLLIFI IINWAAVKAF WGAKASTPEV QSEQSSVRYK DSTSLDQLPT EMPGEDDALS EWNE
Research Areas
Citations (0)

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The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein expressed by this gene is recognized by anti-sperm antibodies from infertile males. Furthermore, antibodies generated against the recombinant protein block in vitro fertilization. This protein localizes to the acrosomal membrane of spermatids and mature spermatozoa where it is thought to play a role in acrosomal morphogenesis and in sperm-egg binding and fusion, respectively.


Yokoyama, K., et al. Nephron Clin Pract 115 (4), C237-C243 (2010) :
Levy, D., et al. BMC Med. Genet. 8 SUPPL 1, S3 (2007) :
Vasan, R.S., et al. BMC Med. Genet. 8 SUPPL 1, S2 (2007) :
Mungall, A.J., et al. Nature 425(6960):805-811(2003)
Hao, Z., et al. Biol. Reprod. 66(3):735-744(2002)

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Cat# AP10775A
(40 western blots)
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