|Application ||WB, IHC-P, FC, E|
|Calculated MW||32143 Da|
|Antigen Region||46-74 aa|
|Other Names||Sperm acrosome membrane-associated protein 1, Sperm acrosomal membrane-associated protein 32, SPACA1, SAMP32|
|Target/Specificity||This SACA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46-74 amino acids from the N-terminal region of human SACA1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SACA1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May be involved in sperm-egg fusion.|
|Cellular Location||Membrane; Single-pass type I membrane protein|
|Tissue Location||Testis specific. At the equatorial segment and the inner acrosomal membrane of capacitated sperm|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein expressed by this gene is recognized by anti-sperm antibodies from infertile males. Furthermore, antibodies generated against the recombinant protein block in vitro fertilization. This protein localizes to the acrosomal membrane of spermatids and mature spermatozoa where it is thought to play a role in acrosomal morphogenesis and in sperm-egg binding and fusion, respectively.
Yokoyama, K., et al. Nephron Clin Pract 115 (4), C237-C243 (2010) :
Levy, D., et al. BMC Med. Genet. 8 SUPPL 1, S3 (2007) :
Vasan, R.S., et al. BMC Med. Genet. 8 SUPPL 1, S2 (2007) :
Mungall, A.J., et al. Nature 425(6960):805-811(2003)
Hao, Z., et al. Biol. Reprod. 66(3):735-744(2002)
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.