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SACA1 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| FC, IHC-P, WB, E |
|---|---|
| Primary Accession | Q9HBV2 |
| Other Accession | NP_112222.1 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 32143 Da |
| Antigen Region | 46-74 aa |
| Gene ID | 81833 |
|---|---|
| Other Names | Sperm acrosome membrane-associated protein 1, Sperm acrosomal membrane-associated protein 32, SPACA1, SAMP32 |
| Target/Specificity | This SACA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46-74 amino acids from the N-terminal region of human SACA1. |
| Dilution | WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | SACA1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | SPACA1 |
|---|---|
| Synonyms | SAMP32 |
| Function | Plays a role in acrosome expansion and establishment of normal sperm morphology during spermatogenesis (By similarity). Important for male fertility (PubMed:11870081). |
| Cellular Location | Cytoplasmic vesicle, secretory vesicle, acrosome inner membrane; Single-pass type I membrane protein. Note=Primarily found in the equatorial segment of the acrosome (PubMed:11870081). The tyrosine phosphorylated protein localizes to a smaller region within the equatorial segment (By similarity). Also expressed weakly in the principal segment (PubMed:11870081). {ECO:0000250|UniProtKB:D5K8A9, ECO:0000269|PubMed:11870081} |
| Tissue Location | Testis specific.. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein expressed by this gene is recognized by anti-sperm antibodies from infertile males. Furthermore, antibodies generated against the recombinant protein block in vitro fertilization. This protein localizes to the acrosomal membrane of spermatids and mature spermatozoa where it is thought to play a role in acrosomal morphogenesis and in sperm-egg binding and fusion, respectively.
References
Yokoyama, K., et al. Nephron Clin Pract 115 (4), C237-C243 (2010) :
Levy, D., et al. BMC Med. Genet. 8 SUPPL 1, S3 (2007) :
Vasan, R.S., et al. BMC Med. Genet. 8 SUPPL 1, S2 (2007) :
Mungall, A.J., et al. Nature 425(6960):805-811(2003)
Hao, Z., et al. Biol. Reprod. 66(3):735-744(2002)
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