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>   home   >   Products   >   Primary Antibodies   >   KAT1 (HAT1) Antibody (C-term)   

KAT1 (HAT1) Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - KAT1 (HAT1) Antibody (C-term) AP1079b
    Western blot analysis of hHAT1-E404 (Cat. #AP1079b) in 293, HepG2, Jurkat cell line lysates (35ug/lane). HAT1 (arrow) was detected using the purified Pab.
    detail
  • IHC-P - KAT1 (HAT1) Antibody (C-term) AP1079b
    Formalin-fixed and paraffin-embedded H.colon tissue reacted with KAT1 (HAT1) Antibody (C-term) (Cat#AP1079b).
    detail
  • IHC-P - KAT1 (HAT1) Antibody (C-term) AP1079b
    Formalin-fixed and paraffin-embedded H.tonsil tissue reacted with KAT1 (HAT1) Antibody (C-term) (Cat#AP1079b).
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession O14929
Other Accession Q5M939, Q8BY71
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 49541 Da
Antigen Region 389-419 aa
Additional Information
Gene ID 8520
Other Names Histone acetyltransferase type B catalytic subunit, Histone acetyltransferase 1, HAT1, KAT1
Target/Specificity This KAT1 (HAT1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 389-419 amino acids from the C-terminal region of human KAT1 (HAT1).
Dilution WB~~1:1000
IHC-P~~1:100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsKAT1 (HAT1) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HAT1
Synonyms KAT1
Function Histone acetyltransferase that plays a role in different biological processes including cell cycle progression, glucose metabolism, histone production or DNA damage repair (PubMed:31278053, PubMed:20953179, PubMed:23653357, PubMed:32081014). Coordinates histone production and acetylation via H4 promoter binding (PubMed:31278053). Acetylates histone H4 at 'Lys-5' (H4K5ac) and 'Lys-12' (H4K12ac) and, to a lesser extent, histone H2A at 'Lys-5' (H2AK5ac) (PubMed:22615379, PubMed:11585814). Drives H4 production by chromatin binding to support chromatin replication and acetylation. Since transcription of H4 genes is tightly coupled to S-phase, plays an important role in S-phase entry and progression (PubMed:31278053). Promotes homologous recombination in DNA repair by facilitating histone turnover and incorporation of acetylated H3.3 at sites of double-strand breaks (PubMed:23653357). In addition, acetylates other substrates such as chromatin-related proteins (PubMed:32081014). Acetylates also RSAD2 which mediates the interaction of ubiquitin ligase UBE4A with RSAD2 leading to RSAD2 ubiquitination and subsequent degradation (PubMed:31812350).
Cellular Location [Isoform A]: Nucleus matrix Mitochondrion
Citations (0)
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Background

Histone acetylation, particularly of histone H4, has been proposed to play an important role in replication-dependent nucleosome assembly. The HAT1 protein contains D, A, and B motifs, which are present in many N-acetyltransferases, including those that acetylate substrates other than histones. The HAT1 holoenzyme consists of 2 subunits: the catalytic 46-kD HAT1 and the accessory p46. The p46 subunit stimulated the activity of HAT1 and bound to core histones. The HAT1 holoenzyme acetylated newly synthesized but not nucleosomal histone H4 at lys5 and lys12, and, to a lesser extent, histone H2A at lys5. HAT1 and p46 polypeptides are located in the nucleus of S-phase cells. HAT1 may play a role in telomeric silencing.

References

Gronroos, E., et al., Mol. Cell 10(3):483-493 (2002).
Makowski, A.M., et al., J. Biol. Chem. 276(47):43499-43502 (2001).
Cheung, P., et al., Mol. Cell 5(6):905-915 (2000).
Verreault, A., et al., Curr. Biol. 8(2):96-108 (1998).

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$ 365.00
$ 140.00
Cat# AP1079b
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