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ACD10 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ACD10 Antibody  (Center) AP10806c
    ACD10 Antibody (Center) (Cat. #AP10806c) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the ACD10 antibody detected the ACD10 protein (arrow).
    detail
  • IHC-P - ACD10 Antibody  (Center) AP10806c
    ACD10 Antibody (Center) (Cat. #AP10806c) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ACD10 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - ACD10 Antibody  (Center) AP10806c
    ACD10 Antibody (Center) (Cat. #AP10806c) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession Q6JQN1
Other Accession NP_079523.3
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 118834 Da
Antigen Region 393-421 aa
Additional Information
Gene ID 80724
Other Names Acyl-CoA dehydrogenase family member 10, ACAD-10, 1399-, ACAD10
Target/Specificity This ACD10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 393-421 amino acids from the Central region of human ACD10.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsACD10 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ACAD10
Function Acyl-CoA dehydrogenase only active with R- and S-2-methyl- C15-CoA.
Tissue Location Widely expressed with highest expression in fetal brain, followed by heart, muscle, kidney and adult brain. Expression levels varying from isoform to isoform.
Research Areas
Citations (0)
citation

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Background

This gene encodes a member of the acyl-CoA dehydrogenase family of enzymes (ACADs), which participate in the beta-oxidation of fatty acids in mitochondria. The encoded enzyme contains a hydrolase domain at the N-terminal portion, a serine/threonine protein kinase catlytic domain in the central region, and a conserved ACAD domain at the C-terminus. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

References

Bian, L., et al. Diabetologia 53(7):1349-1353(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Vega, A., et al. Gynecol. Oncol. 112(1):210-214(2009)
Ye, X., et al. Mol. Biol. Rep. 31(3):191-195(2004)
Sogayar, M.C., et al. Genome Res. 14(7):1413-1423(2004)

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$ 365.00
$ 140.00
Cat# AP10806c
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