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PMAT(Slc29a4) Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - PMAT(Slc29a4) Antibody (C-term) AP1087b
    Western blot analysis of PMAT(Slc29a4) (arrow) using rabbit polyclonal PMAT(Slc29a4) Antibody (C-term) (Cat.#AP1087b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PMAT(Slc29a4) gene (Lane 2) (Origene Technologies).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8R139
Other Accession Q7RTT9
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 58099 Da
Antigen Region 452-481 aa
Additional Information
Gene ID 243328
Other Names Equilibrative nucleoside transporter 4, Solute carrier family 29 member 4, Slc29a4, Ent4
Target/Specificity This PMAT(Slc29a4) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 452-481 amino acids from the C-terminal region of human PMAT(Slc29a4).
Dilution WB~~1:1000
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPMAT(Slc29a4) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Slc29a4
Synonyms Ent4
Function Functions as a polyspecific organic cation transporter, efficiently transporting many organic cations such as monoamine neurotransmitters 1-methyl-4-phenylpyridinium and biogenic amines including serotonin, dopamine, norepinephrine and epinephrine. May play a role in regulating central nervous system homeostasis of monoamine neurotransmitters. May be involved in luminal transport of organic cations in the kidney and seems to use luminal proton gradient to drive organic cation reabsorptprev reabsorption. Does not seem to transport nucleoside and nucleoside analogs such as uridine, cytidine, thymidine, adenosine, inosine, guanosine, and azidothymidine. In (PubMed:16873718) adenosine is efficiently transported but in a fashion highly sensitive to extracellular pH, with maximal activity in the pH range 5.5 to 6.5. Glu-206 is essential for the cation selectivity and may function as the charge sensor for cationic substrates. Transport is chloride and sodium-independent but appears to be sensitive to changes in membrane potential. Weakly inhibited by the classical inhibitors of equilibrative nucleoside transport, dipyridamole, dilazep, and nitrobenzylthioinosine. May play a role in the regulation of extracellular adenosine concentrations in cardiac tissues, in particular during ischemia (By similarity).
Cellular Location Cell membrane; Multi-pass membrane protein. Apical cell membrane; Multi-pass membrane protein Note=Located to the plasma membranes of ventricular myocytes and vascular endothelial cells. Targeted to the apical membranes of differentiated kidney epithelial cells (By similarity)
Tissue Location Expressed in heart.
Research Areas
Citations (0)

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PMAT(Slc29a4) is a member of the SLC29 family and encodes a plasma membrane protein with 11 transmembrane helices. This protein catalyzes the re-uptake of monoamines into presynaptic neurons, thus determining the intensity and duration of monoamine neural signaling. It has been shown to transport several compounds, including serotonin, dopamine, and the neurotoxin 1-methyl-4-phenylpyridinium.


Barnes,K., Circ. Res. 99 (5), 510-519 (2006)
Engel,K., J. Biol. Chem. 279 (48), 50042-50049 (2004)

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Cat# AP1087b
(40 western blots)
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