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SOHLH1 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - SOHLH1 Antibody (N-term) AP10919A
    SOHLH1 Antibody (N-term) (Cat. #AP10919a) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the SOHLH1 antibody detected the SOHLH1 protein (arrow).
    detail
  • IHC-P - SOHLH1 Antibody (N-term) AP10919A
    SOHLH1 Antibody (N-term) (Cat. #AP10919a) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SOHLH1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - SOHLH1 Antibody (N-term) AP10919A
    SOHLH1 Antibody (N-term) (Cat. #AP10919a) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession Q5JUK2
Other Accession NP_001012415.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 34526 Da
Antigen Region 40-68 aa
Additional Information
Gene ID 402381
Other Names Spermatogenesis- and oogenesis-specific basic helix-loop-helix-containing protein 1, SOHLH1, C9orf157, NOHLH, TEB2
Target/Specificity This SOHLH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40-68 amino acids from the N-terminal region of human SOHLH1.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSOHLH1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SOHLH1
Synonyms C9orf157, NOHLH, TEB2
Function Transcription regulator of both male and female germline differentiation. Suppresses genes involved in spermatogonial stem cells maintenance, and induces genes important for spermatogonial differentiation. Coordinates oocyte differentiation without affecting meiosis I (By similarity).
Cellular Location Cytoplasm {ECO:0000250|UniProtKB:Q6IUP1}. Nucleus {ECO:0000250|UniProtKB:Q6IUP1, ECO:0000255|PROSITE-ProRule:PRU00981}
Research Areas
Citations (0)
citation

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Background

Probable transcription factor required during spermatogenesis and oogenesis (By similarity).

References

Choi, Y., et al. Hum. Mutat. 31(7):788-793(2010)
Pangas, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 103(21):8090-8095(2006)

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$ 182.50
$ 70.00
Cat# AP10919A
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