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COMT Antibody (N-term)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP10956a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP10956a-ev20 | 20 ug 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
COMT Antibody (N-term) - Product info | |
| Application | WB, IHC
|
| Primary Accession | P21964 |
| Other Accession | NP_001128634.1 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 30037 Da |
COMT Antibody (N-term) - Additional info | |
| Gene ID 1312 | |
| Other Names COMT; Catechol O-methyltransferase | |
| Target/Specificity This COMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 37-65 amino acids from the N-terminal region of human COMT. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:50~100 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions COMT Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
COMT Antibody (N-term) - Protein Information | |
| Name COMT | |
| Function Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol | |
| Cellular Location Isoform Soluble: Cytoplasm. | |
| Tissue Location Brain, liver, placenta, lymphocytes and erythrocytes | |
COMT Antibody (N-term) - Related products
AP10956a: COMT Antibody (N-term)
DC03243: Human COMT cDNA Clone
LY10302a: COMT Over-expression Lysate
AT1588a: COMT Antibody (monoclonal) (M01)
COMT Antibody (N-term) - Application data
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Western blot analysis of COMT (arrow) using rabbit polyclonal COMT Antibody (N-term) (Cat. #AP10956a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the COMT gene (Lane 2) .
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COMT antibody (N-term) (Cat. #AP10956a) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the COMT antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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COMT Antibody (N-term) (Cat. #AP10956a) western blot analysis in K562,MCF-7 cell line lysates (35ug/lane).This demonstrates the COMT antibody detected the COMT protein (arrow).
COMT Antibody (N-term) - Research Areas
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BACKGROUND
Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters.
REFERENCES
Paloyelis, Y., et al. Neuropsychopharmacology 35(12):2414-2426(2010) Stroth, S., et al. Neurobiol Learn Mem 94(3):364-372(2010) Lim, J.H., et al. Pharmacogenet. Genomics 20(10):605-610(2010) Demetrovics, Z., et al. Compr Psychiatry 51(5):510-515(2010) Bodenmann, S., et al. Sleep 33(8):1027-1035(2010)
