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FH Antibody (N-term)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP10963a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP10963a-ev20 | Ev20 size 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
FH Antibody (N-term) - Product info | |
| Application | WB, IHC, FC, IF
|
| Primary Accession | P07954 |
| Other Accession | P14408, P10173, Q60HF9, NP_000134.2 |
| Reactivity | Human, Mouse |
| Predicted | Pig, Monkey, Rat |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 54637 Da |
FH Antibody (N-term) - Additional info | |
| Gene ID 2271 | |
| Other Names FH; Fumarate hydratase, mitochondrial | |
| Target/Specificity This FH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 35-63 amino acids from the N-terminal region of human FH. | |
| Dilution WB~~1:1000WB~~1:1000 IHC~~1:50~100 FC~~1:10~50 IF~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions FH Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
FH Antibody (N-term) - Protein Information | |
| Name FH | |
| Function Also acts as a tumor suppressor. | |
| Cellular Location Isoform Mitochondrial: Mitochondrion. | |
| Tissue Location Expressed in red blood cells; underexpressed in red blood cells (cytoplasm) of patients with hereditary non- spherocytic hemolytic anemia of unknown etiology | |
FH Antibody (N-term) - Related products
AM2120a: FH Antibody (N-term) (Ascites)
AP10963a: FH Antibody (N-term)
AP18400a: FH Antibody (N-term)
LY12566a: FH Over-expression Lysate
AT2044a: FH Antibody (monoclonal) (M07)
FH Antibody (N-term) - Application data
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FH Antibody (N-term) (Cat. #AP10963a) western blot analysis in Hela cell line and mouse kidney tissue lysates (35ug/lane).This demonstrates the FH antibody detected the FH protein (arrow).
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FH antibody (N-term) (Cat. #AP10963a) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FH antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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FH Antibody (N-term) (Cat. #AP10963a) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Confocal immunofluorescent analysis of FH Antibody (N-term) (Cat. #AP10963a) with Hela cell followed by Alexa Fluor庐 489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
FH Antibody (N-term) - Research Areas
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BACKGROUND
The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy.
REFERENCES
Shimada, M., et al. Hum. Genet. 128(4):433-441(2010) Allegri, G., et al. J. Inherit. Metab. Dis. 33(4):411-419(2010) Yogev, O., et al. PLoS Biol. 8 (3), E1000328 (2010) : Yang, Y., et al. Cancer Genet. Cytogenet. 196(1):45-55(2010) Rikova, K., et al. Cell 131(6):1190-1203(2007)
