|Application ||WB, FC, E|
|Calculated MW||85077 Da|
|Antigen Region||156-185 aa|
|Other Names||Anaphase-promoting complex subunit 5, APC5, Cyclosome subunit 5, ANAPC5, APC5|
|Target/Specificity||This ANAPC5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156-185 amino acids from the Central region of human ANAPC5.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ANAPC5 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene. These differences cause translation initiation at a downstream AUG and result in a shorter protein (isoform b), compared to isoform a.
Wasch, R., et al. Oncogene 29(1):1-10(2010)
Jin, L., et al. Cell 133(4):653-665(2008)
Liu, J., et al. Cancer Biol. Ther. 5(7):760-762(2006)
Dube, P., et al. Mol. Cell 20(6):867-879(2005)
Turnell, A.S., et al. Nature 438(7068):690-695(2005)
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