|Application ||WB, E|
|Other Accession||NP_001138820.1, NP_079049.2|
|Calculated MW||47270 Da|
|Antigen Region||36-63 aa|
|Other Names||Lysine-specific demethylase 8, JmjC domain-containing protein 5, Jumonji domain-containing protein 5, KDM8, JMJD5|
|Target/Specificity||This JMJD5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36-63 amino acids from the N-terminal region of human JMJD5.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||JMJD5 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Histone demethylase required for G2/M phase cell cycle progression (PubMed:20457893). Specifically demethylates dimethylated 'Lys-36' (H3K36me2) of histone H3, an epigenetic repressive mark, thereby acting as a transcription activator (PubMed:20457893). Regulates expression of CCNA1 (cyclin-A1), leading to regulate cancer cell proliferation (PubMed:20457893). In a complex with coregulator RCCD1, is also able to demethylate trimethylated 'Lys-36' (H3K36me3) of histone H3 (PubMed:24981860). Plays a role in transcriptional repression of satellite repeats, possibly by regulating H3K36 methylation levels in centromeric regions together with RCCD1 (PubMed:24981860). Possibly together with RCCD1, involved in proper mitotic spindle organization and chromosome segregation (PubMed:24981860). Plays a role in regulating alpha-tubulin acetylation and cytoskeletal microtubule stability (PubMed:28455245). Might function as a protein hydroxylase (PubMed:22851697, PubMed:24100311). Under stress conditions that cause a DNA damage response, acts as a histone protease by cleaving the N-terminal tail of histone H3 at the carboxyl side of monomethyl-lysine (Kme1) residues, preferably at monomethylated 'Lys-9' (H3K9me1) (PubMed:28982940). The histone variant H3F3A is the major target for cleavage (PubMed:28982940).|
|Cellular Location||Nucleus. Chromosome. Note=Colocalizes with trimethylated 'Lys-9' of histone H3 (H3K9me3).|
|Tissue Location||Weakly expressed in most cells. Highly expressed in breast cancer cells.|
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Provided below are standard protocols that you may find useful for product applications.
This gene likely encodes a histone lysine demethylase. Studies of a similar protein in mouse indicate a potential role for this protein as a tumor suppressor. Alternatively spliced transcript variants have been described.
Hsia, D.A., et al. Proc. Natl. Acad. Sci. U.S.A. 107(21):9671-9676(2010)
Shi, Y. Nat. Rev. Genet. 8(11):829-833(2007)
Suzuki, T., et al. EMBO J. 25(14):3422-3431(2006)
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