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>   home   >   Products   >   Primary Antibodies   >   RT34 Antibody (N-term)   

RT34 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - RT34 Antibody (N-term) AP10991a
    RT34 Antibody (N-term) (Cat. #AP10991a) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the RT34 antibody detected the RT34 protein (arrow).
  • FC - RT34 Antibody (N-term) AP10991a
    RT34 Antibody (N-term) (Cat. #AP10991a) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession P82930
Other Accession NP_076425.1
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Antigen Region 4-32 aa
Additional Information
Other Names 28S ribosomal protein S34, mitochondrial, MRP-S34, S34mt, MRPS34
Target/Specificity This RT34 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 4-32 amino acids from the N-terminal region of human RT34.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRT34 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MRPS34
Function Required for mitochondrial translation, plays a role in maintaining the stability of the small ribosomal subunit and the 12S rRNA that are required for mitoribosome formation.
Cellular Location Mitochondrion. EMBL; BC001182; AAH01182.1; -; mRNA CCDS; CCDS10444.1; - RefSeq; NP_001287829.1; NM_001300900.1 RefSeq; NP_076425.1; NM_023936.1 UniGene; Hs.720388; - PDB; 3J9M; EM; 3.50 A; A0=1-218 PDBsum; 3J9M; - ProteinModelPortal; P82930; - BioGrid; 122442; 78 IntAct; P82930; 10 STRING; 9606.ENSP00000380531; - iPTMnet; P82930; - PhosphoSitePlus; P82930; - SwissPalm; P82930; - BioMuta; MRPS34; - DMDM; 24212369; - EPD; P82930; - MaxQB; P82930; - PaxDb; P82930; - PeptideAtlas; P82930; - PRIDE; P82930; - TopDownProteomics; P82930; - DNASU; 65993; - Ensembl; ENST00000397375; ENSP00000380531; ENSG00000074071 GeneID; 65993; - KEGG; hsa:65993; - UCSC; uc002cmo.4; human CTD; 65993; - GeneCards; MRPS34; - HGNC; HGNC:16618; MRPS34 HPA; HPA042112; - MIM; 611994; gene neXtProt; NX_P82930; - OpenTargets; ENSG00000074071; - PharmGKB; PA31021; - eggNOG; ENOG410IGV1; Eukaryota eggNOG; ENOG4111FKH; LUCA GeneTree; ENSGT00390000008964; - HOGENOM; HOG000154216; - HOVERGEN; HBG036130; - InParanoid; P82930; - KO; K17412; - PhylomeDB; P82930; - TreeFam; TF320386; - BioCyc; ZFISH:ENSG00000074071-MONOMER; - Reactome; R-HSA-5368286; Mitochondrial translation initiation Reactome; R-HSA-5389840; Mitochondrial translation elongation Reactome; R-HSA-5419276; Mitochondrial translation termination ChiTaRS; MRPS34; human GenomeRNAi; 65993; - PRO; PR:P82930; - Proteomes; UP000005640; Chromosome 16 Bgee; ENSG00000074071; - CleanEx; HS_MRPS34; - ExpressionAtlas; P82930; baseline and differential Genevisible; P82930; HS GO; GO:0005743; C:mitochondrial inner membrane; TAS:Reactome GO; GO:0005739; C:mitochondrion; IDA:HPA GO; GO:0005840; C:ribosome; IEA:UniProtKB-KW GO; GO:0003735; F:structural constituent of ribosome; IEA:InterPro GO; GO:0032543; P:mitochondrial translation; ISS:UniProtKB GO; GO:0070125; P:mitochondrial translational elongation; TAS:Reactome GO; GO:0070126; P:mitochondrial translational termination; TAS:Reactome InterPro; IPR032053; MRP-S34 Pfam; PF16053; MRP-S34; 1 1: Evidence at protein level; 3D-structure; Complete proteome; Mitochondrion; Polymorphism; Reference proteome; Ribonucleoprotein; Ribosomal protein INIT_MET 1 1 Removed. {ECO:0000250|UniProtKB:P82929} CHAIN 2 218 28S ribosomal protein S34, mitochondrial /FTId=PRO_0000087730 VARIANT 33 33 L -> I (in dbSNP:rs11552431) /FTId=VAR_052050 SEQUENCE 218 AA; 25650 MW; 58DC48FF63276060 CRC64; MARKKVRPRL IAELARRVRA LREQLNRPRD SQLYAVDYET LTRPFSGRRL PVRAWADVRR ESRLLQLLGR LPLFGLGRLV TRKSWLWQHD EPCYWRLTRV RPDYTAQNLD HGKAWGILTF KGKTESEARE IEHVMYHDWR LVPKHEEEAF TAFTPAPEDS LASVPYPPLL RAMIIAERQK NGDTSTEEPM LNVQRIRMEP WDYPAKQEDK GRAKGTPV
Citations (0)

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Background

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. Alternate splice variants of this gene have been described but their full-length nature has not been determined.

References

Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Zhang, Z., et al. Genomics 81(5):468-480(2003)
Ogawa, F., et al. Biochem. Biophys. Res. Commun. 300(3):789-792(2003)
Cavdar Koc, E., et al. J. Biol. Chem. 276(22):19363-19374(2001)
O'Brien, T.W., et al. J. Biol. Chem. 275(24):18153-18159(2000)

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$ 295.00
$ 99.00
Cat# AP10991a
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(40 western blots)
Availability: In Stock
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