DC12 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | Q96FZ2 |
Other Accession | NP_064572.2, NP_001006109.1 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 40575 Da |
Antigen Region | 281-310 aa |
Gene ID | 56941 |
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Other Names | Embryonic stem cell-specific 5-hydroxymethylcytosine-binding protein, ES cell-specific 5hmC-binding protein, Putative peptidase SRAPD1, 34--, SRAP domain-containing protein 1, HMCES, C3orf37, DC12, SRAPD1 |
Target/Specificity | This DC12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 281-310 amino acids from the C-terminal region of human DC12. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | DC12 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | HMCES {ECO:0000303|PubMed:30554877, ECO:0000312|HGNC:HGNC:24446} |
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Function | Sensor of abasic sites in single-stranded DNA (ssDNA) required to preserve genome integrity by promoting error-free repair of abasic sites (PubMed:30554877, PubMed:31235915, PubMed:31235913). Acts as an enzyme that recognizes and binds abasic sites in ssDNA at replication forks and chemically modifies the lesion by forming a covalent cross-link with DNA: forms a stable thiazolidine linkage between a ring-opened abasic site and the alpha-amino and sulfhydryl substituents of its N-terminal catalytic cysteine residue (PubMed:30554877, PubMed:31235913). The HMCES DNA-protein cross-link is then degraded by the proteasome (PubMed:30554877). Promotes error-free repair of abasic sites by acting as a 'suicide' enzyme that is degraded, thereby protecting abasic sites from translesion synthesis (TLS) polymerases and endonucleases that are error-prone and would generate mutations and double-strand breaks (PubMed:30554877). Has preference for ssDNA, but can also accommodate double-stranded DNA with 3' or 5' overhang (dsDNA), and dsDNA-ssDNA 3' junction (PubMed:31235915, PubMed:31806351). Also involved in class switch recombination (CSR) in B-cells independently of the formation of a DNA- protein cross-link: acts by binding and protecting ssDNA overhangs to promote DNA double-strand break repair through the microhomology- mediated alternative-end-joining (Alt-EJ) pathway (By similarity). Acts as a protease: mediates autocatalytic processing of its N-terminal methionine in order to expose the catalytic cysteine (By similarity). |
Cellular Location | Chromosome. Note=Recruited to chromatin following DNA damage (PubMed:30554877) Localizes to replication forks (PubMed:30554877) |
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Provided below are standard protocols that you may find useful for product applications.
References
Gerhard, D.S., et al. Genome Res. 14 (10B), 2121-2127 (2004) :
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