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NUSAP Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - NUSAP Antibody  (N-term) AP11000a
    NUSAP Antibody (N-term) (Cat. #AP11000a) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the NUSAP antibody detected the NUSAP protein (arrow).
  • IHC-P - NUSAP Antibody  (N-term) AP11000a
    NUSAP antibody (N-term) (Cat. #AP11000a) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NUSAP antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9BXS6
Other Accession NP_057443.2, NP_001123369.1, NP_060924.4
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 49452 Da
Antigen Region 27-56 aa
Additional Information
Gene ID 51203
Other Names Nucleolar and spindle-associated protein 1, NuSAP, NUSAP1, ANKT
Target/Specificity This NUSAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-56 amino acids from the N-terminal region of human NUSAP.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNUSAP Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms ANKT
Function Microtubule-associated protein with the capacity to bundle and stabilize microtubules (By similarity). May associate with chromosomes and promote the organization of mitotic spindle microtubules around them.
Cellular Location Cytoplasm. Nucleus, nucleolus. Cytoplasm, cytoskeleton, spindle Chromosome. Note=Found in the cytoplasm and nucleolus during interphase and redistributes to the mitotic spindle in prometaphase (By similarity). Localizes to the mitotic spindle during anaphase and telophase then disappears from around the chromosomes during cytokinesis (By similarity). Localizes to multiple distinct regions of chromosomes throughout mitosis {ECO:0000250, ECO:0000269|PubMed:17276916, ECO:0000269|PubMed:17618083}
Research Areas
Citations (0)

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NUSAP1 is a nucleolar-spindle-associated protein that plays a role in spindle microtubule organization (Raemaekers et al., 2003 [PubMed 12963707]).


Wadia, P.P., et al. Blood 115(10):2077-2087(2010)
Matsuoka, S., et al. Science 316(5828):1160-1166(2007)
Nousiainen, M., et al. Proc. Natl. Acad. Sci. U.S.A. 103(14):5391-5396(2006)
Raemaekers, T., et al. J. Cell Biol. 162(6):1017-1029(2003)

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$ 295.00
$ 99.00
Cat# AP11000a
(40 western blots)
Availability: In Stock
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