HDAC2 Antibody (C-term)Purified Rabbit Polyclonal Antibody (Pab)

Western blot analysis of HDAC2 (arrow) using rabbit polyclonal HDAC2 Antibody (C-term) (Cat.#AP1102b).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HDAC2 gene (Lane 2) (Origene Technologies).

Western blot analysis of anti-HDAC2 Antibody (C-term) (Cat.#AP1102b) in 293 cell line lysates (35ug/lane). HDAC2(arrow) was detected using the purified Pab.

Western blot analysis of anti-HDAC2 Antibody (C-term) (Cat.#AP1102b) in mouse spleen tissue lysates (35ug/lane). HDAC2(arrow) was detected using the purified Pab.

Confocal immunofluorescent analysis of HDAC2 Antibody (C-term)(Cat#AP1102b) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

HDAC2 Antibody (C-term) (Cat. #AP1102b)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HDAC2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Fluorescent confocal image of Hela cell stained with HDAC2 Antibody (C-term)(Cat#AP1102b).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HDAC2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). hHDAC2 immunoreactivity is localized to Nucleus significantly.