|Application ||WB, IHC-P, IF, FC, E|
|Calculated MW||28058 Da|
|Antigen Region||56-83 aa|
|Other Names||Nuclear receptor subfamily 0 group B member 2, Orphan nuclear receptor SHP, Small heterodimer partner, NR0B2, SHP|
|Target/Specificity||This NR0B2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-83 amino acids from the Central region of human NR0B2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||NR0B2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Acts as a transcriptional regulator. Acts as a negative regulator of receptor-dependent signaling pathways. Specifically inhibits transactivation of the nuclear receptor with whom it interacts. Inhibits transcriptional activity of NEUROD1 on E-box- containing promoter by interfering with the coactivation function of the p300/CBP-mediated trancription complex for NEUROD1.|
|Cellular Location||Nucleus. Cytoplasm. Note=Colocalizes with NEUROD1 in the nucleus|
|Tissue Location||Liver. Low levels of expression were detected in heart and pancreas.|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is an unusual orphan receptor that contains a putative ligand-binding domain but lacks a conventional DNA-binding domain. The gene product is a member of the nuclear hormone receptor family, a group of transcription factors regulated by small hydrophobic hormones, a subset of which do not have known ligands and are referred to as orphan nuclear receptors. The protein has been shown to interact with retinoid and thyroid hormone receptors, inhibiting their ligand-dependent transcriptional activation. In addition, interaction with estrogen receptors has been demonstrated, leading to inhibition of function. Studies suggest that the protein represses nuclear hormone receptor-mediated transactivation via two separate steps: competition with coactivators and the direct effects of its transcriptional repressor function.
Zhou, T., et al. J. Biol. Chem. 285(32):24871-24881(2010) Roberts, K.E., et al. Gastroenterology 139(1):130-139(2010) Kim, K., et al. Biochem. J. 427(3):413-422(2010) Yang, Z., et al. J. Int. Med. Res. 38(1):142-149(2010) Ondracek, C.R., et al. J. Virol. 83(23):12535-12544(2009)
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