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DARS Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - DARS Antibody (N-term) AP11088a
    DARS Antibody (N-term) (Cat. #AP11088a) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the DARS antibody detected the DARS protein (arrow).
  • WB - DARS Antibody (N-term) AP11088a
    Western blot analysis of DARS (arrow) using rabbit polyclonal DARS Antibody (N-term) (Cat. #AP11088a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DARS gene.
  • IHC-P - DARS Antibody (N-term) AP11088a
    DARS Antibody (N-term) (Cat. #AP11088a)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DARS Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • IF - DARS Antibody (N-term) AP11088a
    Confocal immunofluorescent analysis of DARS Antibody (N-term)(Cat#AP11088a) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
  • FC - DARS Antibody (N-term) AP11088a
    DARS Antibody (N-term) (Cat. #AP11088a) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P14868
Other Accession Q3SYZ4, NP_001340.2
Reactivity Human
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 57136 Da
Antigen Region 154-183 aa
Additional Information
Gene ID 1615
Other Names Aspartate--tRNA ligase, cytoplasmic, Aspartyl-tRNA synthetase, AspRS, Cell proliferation-inducing gene 40 protein, DARS
Target/Specificity This DARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154-183 amino acids from the N-terminal region of human DARS.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDARS Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.
Cellular Location Cytoplasm.
Tissue Location Expression in the developing and adult brain shows similar patterns. Highly expressed in the ventricular and subventricular zones, including hippocampal subfields, the midlateral temporaal cortex and the frontal polar cortex. The cerebellum, cereral cortex, hippocampus, and lateral ventricle show preferential neuronal expression. Expression in the peripheral neurons is evident in the colon
Citations (0)

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Aspartyl-tRNA synthetase (DARS) is part of a multienzyme complex of aminoacyl-tRNA synthetases. Aspartyl-tRNA synthetase charges its cognate tRNA with aspartate during protein biosynthesis.


Wu, C., et al. Proteomics 7(11):1775-1785(2007)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Tu, L.C., et al. Mol. Cell Proteomics 6(4):575-588(2007)
Ling, C., et al. J. Biol. Chem. 280(41):34755-34763(2005)
Bonnefond, L., et al. Biochemistry 44(12):4805-4816(2005)

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$ 295.00
$ 99.00
Cat# AP11088a
(40 western blots)
Availability: In Stock
Bulk Size
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