|Application ||WB, E|
|Other Accession||NP_001198.2, NP_001032726.1|
|Calculated MW||22168 Da|
|Antigen Region||1-30 aa|
|Other Names||Transcription factor BTF3, Nascent polypeptide-associated complex subunit beta, NAC-beta, RNA polymerase B transcription factor 3, BTF3, NACB|
|Target/Specificity||This BTF3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human BTF3.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||BTF3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||When associated with NACA, prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. BTF3 is also a general transcription factor that can form a stable complex with RNA polymerase II. Required for the initiation of transcription.|
|Cellular Location||Cytoplasm. Nucleus. Note=The heterodimer with NACA is cytoplasmic|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes the basic transcription factor 3. This protein forms a stable complex with RNA polymerase IIB and is required for transcriptional initiation. Alternative splicing results in multiple transcript variants encoding different isoforms. This gene has multiple pseudogenes.
Green, C.D., et al. Mol. Cancer Res. 5(11):1191-1200(2007)
Matsuoka, S., et al. Science 316(5828):1160-1166(2007)
Kusumawidjaja, G., et al. Cancer Biol. Ther. 6(3):367-376(2007)
Freire, M.A. Gene 345(2):271-277(2005)
Harrington, J.J., et al. Nat. Biotechnol. 19(5):440-445(2001)
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