- CITATIONS: 2
|Application ||WB, IHC-P, IF, E|
|Calculated MW||56278 Da|
|Antigen Region||235-263 aa|
|Other Names||Cytochrome P450 2B6, 11413-, 4-cineole 2-exo-monooxygenase, CYPIIB6, Cytochrome P450 IIB1, CYP2B6|
|Target/Specificity||This CYP2B6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 235-263 amino acids from the Central region of human CYP2B6.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CYP2B6 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Acts as a 1,4-cineole 2-exo-monooxygenase.|
|Cellular Location||Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein|
|Tissue Location||Expressed in liver, lung and heart right ventricle.|
Provided below are standard protocols that you may find useful for product applications.
This gene, CYP2B6, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to metabolize some xenobiotics, such as the anti-cancer drugs cyclophosphamide and ifosphamide. Transcript variants for this gene have been described; however, it has not been resolved whether these transcripts are in fact produced by this gene or by a closely related pseudogene, CYP2B7. Both the gene and the pseudogene are located in the middle of a CYP2A pseudogene found in a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q.
Figueroa, S.C., et al. Ther Drug Monit 32(5):579-585(2010)
Chou, M., et al. Antimicrob. Agents Chemother. 54(10):4432-4439(2010)
Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)
Bunten, H., et al. Clin. Pharmacol. Ther. 88(3):383-389(2010)
Gounden, V., et al. AIDS Res Ther 7, 32 (2010) :
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