DDX27 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, FC, E |
---|---|
Primary Accession | Q96GQ7 |
Other Accession | A1A4H6, NP_060365.7 |
Reactivity | Human |
Predicted | Bovine |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 89835 Da |
Antigen Region | 770-796 aa |
Gene ID | 55661 |
---|---|
Other Names | Probable ATP-dependent RNA helicase DDX27, DEAD box protein 27, DDX27, RHLP |
Target/Specificity | This DDX27 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 770-796 amino acids from the C-terminal region of human DDX27. |
Dilution | WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | DDX27 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | DDX27 |
---|---|
Synonyms | cPERP-F {ECO:0000303|PubMed:20813266}, R |
Function | Probable ATP-dependent RNA helicase. Component of the nucleolar ribosomal RNA (rRNA) processing machinery that regulates 3' end formation of ribosomal 47S rRNA (PubMed:25825154). |
Cellular Location | Nucleus, nucleolus. Chromosome. Note=Associates with 60S and 90S pre-ribosomal particles (PubMed:25825154) |
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Provided below are standard protocols that you may find useful for product applications.
Background
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, the function of which has not been determined. [provided by RefSeq].
References
Deloukas, P., et al. Nature 414(6866):865-871(2001)
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