IFI35 Antibody (N-term R30)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, E |
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Primary Accession | P80217 |
Other Accession | NP_005524 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 31546 Da |
Antigen Region | 15-42 aa |
Gene ID | 3430 |
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Other Names | Interferon-induced 35 kDa protein, IFP 35, Ifi-35, IFI35, IFP35 |
Target/Specificity | This IFI35 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-42 amino acids from the N-terminal region of human IFI35. |
Dilution | WB~~1:1000 IHC-P~~1:50~100 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | IFI35 Antibody (N-term R30) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | IFI35 (HGNC:5399) |
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Function | Acts as a signaling pathway regulator involved in innate immune system response (PubMed:26342464, PubMed:29038465, PubMed:29350881). In response to interferon IFN-alpha, associates in a complex with signaling pathway regulator NMI to regulate immune response; the complex formation prevents proteasome-mediated degradation of IFI35 and correlates with IFI35 dephosphorylation (PubMed:10779520, PubMed:10950963). In complex with NMI, inhibits virus-triggered type I interferon/IFN-beta production (PubMed:26342464). In complex with NMI, negatively regulates nuclear factor NF-kappa-B signaling by inhibiting the nuclear translocation, activation and transcription of the NF-kappa-B subunit p65/RELA, resulting in the inhibition of endothelial cell proliferation, migration and re-endothelialization of injured arteries (PubMed:29350881). Beside its role as an intracellular signaling pathway regulator, also functions extracellularly as damage-associated molecular patterns (DAMPs) to promote inflammation when actively released by macrophage to the extracellular space during cell injury and pathogen invasion (PubMed:29038465). Macrophage-secreted IFI35 activates NF-kappa-B signaling in adjacent macrophages through Toll- like receptor 4/TLR4 activation, thereby inducing NF-kappa-B translocation from the cytoplasm into the nucleus which promotes the release of pro-inflammatory cytokines (PubMed:29038465). |
Cellular Location | Cytoplasm. Nucleus. Secreted Note=Cytoplasmic IFI35 localizes in punctate granular structures (PubMed:10950963). Nuclear localization increased is stimulated by IFN- alpha (PubMed:8288566, PubMed:10950963). Extracelullar following secretion by macrophage (PubMed:29038465) |
Tissue Location | Expressed in a wide range of cell types, including fibroblasts, macrophages, and epithelial cells |
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Provided below are standard protocols that you may find useful for product applications.
Background
Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.
References
Johnatty, S.E., et al. PLoS Genet. 6 (7), E1001016 (2010) :
Wang, J., et al. J. Proteome Res. 7(9):3879-3889(2008)
Tan, J., et al. J. Virol. 82(9):4275-4283(2008)
Zhang, L., et al. Cell. Signal. 19(5):932-944(2007)
Oh, J.H., et al. Mamm. Genome 16(12):942-954(2005)
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