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EMG1 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - EMG1 Antibody (Center) AP11129c
    EMG1 Antibody (Center) (Cat. #AP11129c) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the EMG1 antibody detected the EMG1 protein (arrow).
  • FC - EMG1 Antibody (Center) AP11129c
    EMG1 Antibody (Center) (Cat. #AP11129c) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q92979
Other Accession O35130, NP_006322.4
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 26720 Da
Antigen Region 104-133 aa
Additional Information
Gene ID 10436
Other Names Ribosomal RNA small subunit methyltransferase NEP1, 211-, 18S rRNA (pseudouridine(1248)-N1)-methyltransferase, 18S rRNA Psi1248 methyltransferase, Nucleolar protein EMG1 homolog, Protein C2f, Ribosome biogenesis protein NEP1, EMG1 {ECO:0000303|PubMed:19463982}
Target/Specificity This EMG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-133 amino acids from the Central region of human EMG1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEMG1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EMG1 {ECO:0000303|PubMed:19463982}
Function S-adenosyl-L-methionine-dependent pseudouridine N(1)- methyltransferase that methylates pseudouridine at position 1248 (Psi1248) in 18S rRNA. Involved the biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi) conserved in eukaryotic 18S rRNA. Is not able to methylate uridine at this position (PubMed:20047967). Has also an essential role in 40S ribosomal subunit biogenesis independent on its methyltransferase activity, facilitating the incorporation of ribosomal protein S19 during the formation of pre-ribosomes (By similarity).
Cellular Location Nucleus, nucleolus
Citations (0)

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This gene encodes an essential, conserved eukaryotic protein involved in ribosome biogenesis. In yeast, the related protein is a component of the small subunit processome and is essential for biogenesis of the ribosomal 40S subunit. A mutation in this gene has been associated with Bowen-Conradi syndrome.


Armistead, J., et al. Am. J. Hum. Genet. 84(6):728-739(2009)
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Lamont, R.E., et al. Am. J. Med. Genet. A 132A (2), 136-143 (2005) :
Bernstein, K.A., et al. Eukaryotic Cell 3(6):1619-1626(2004)
Eschrich, D., et al. Curr. Genet. 40(5):326-338(2002)

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$ 295.00
$ 99.00
Cat# AP11129c
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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