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EMG1 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, FC, E |
|---|---|
| Primary Accession | Q92979 |
| Other Accession | O35130, NP_006322.4 |
| Reactivity | Human |
| Predicted | Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 26720 Da |
| Antigen Region | 104-133 aa |
| Gene ID | 10436 |
|---|---|
| Other Names | Ribosomal RNA small subunit methyltransferase NEP1, 211-, 18S rRNA (pseudouridine(1248)-N1)-methyltransferase, 18S rRNA Psi1248 methyltransferase, Nucleolar protein EMG1 homolog, Protein C2f, Ribosome biogenesis protein NEP1, EMG1 {ECO:0000303|PubMed:19463982} |
| Target/Specificity | This EMG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-133 amino acids from the Central region of human EMG1. |
| Dilution | WB~~1:1000 FC~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | EMG1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | EMG1 {ECO:0000303|PubMed:19463982} |
|---|---|
| Function | S-adenosyl-L-methionine-dependent pseudouridine N(1)- methyltransferase that methylates pseudouridine at position 1248 (Psi1248) in 18S rRNA. Involved the biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi) conserved in eukaryotic 18S rRNA. Is not able to methylate uridine at this position (PubMed:20047967). Has also an essential role in 40S ribosomal subunit biogenesis independent on its methyltransferase activity, facilitating the incorporation of ribosomal protein S19 during the formation of pre-ribosomes (By similarity). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed:34516797). |
| Cellular Location | Nucleus, nucleolus |
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes an essential, conserved eukaryotic protein involved in ribosome biogenesis. In yeast, the related protein is a component of the small subunit processome and is essential for biogenesis of the ribosomal 40S subunit. A mutation in this gene has been associated with Bowen-Conradi syndrome.
References
Armistead, J., et al. Am. J. Hum. Genet. 84(6):728-739(2009)
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Lamont, R.E., et al. Am. J. Med. Genet. A 132A (2), 136-143 (2005) :
Bernstein, K.A., et al. Eukaryotic Cell 3(6):1619-1626(2004)
Eschrich, D., et al. Curr. Genet. 40(5):326-338(2002)
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