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ELP3 Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q9H9T3 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 62259 Da |
| Antigen Region | 9-44 aa |
| Gene ID | 55140 |
|---|---|
| Other Names | Elongator complex protein 3, hELP3, ELP3 |
| Target/Specificity | This ELP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-44 amino acids from the N-terminal region of human ELP3. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | ELP3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ELP3 {ECO:0000303|PubMed:15902492, ECO:0000312|HGNC:HGNC:20696} |
|---|---|
| Function | Catalytic tRNA acetyltransferase subunit of the elongator complex which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5- methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine) (PubMed:29415125). In the elongator complex, acts as a tRNA uridine(34) acetyltransferase by mediating formation of carboxymethyluridine in the wobble base at position 34 in tRNAs (By similarity). May also act as a protein lysine acetyltransferase by mediating acetylation of target proteins; such activity is however unclear in vivo and recent evidences suggest that ELP3 primarily acts as a tRNA acetyltransferase (PubMed:29415125). Involved in neurogenesis: regulates the migration and branching of projection neurons in the developing cerebral cortex, through a process depending on alpha-tubulin acetylation (PubMed:19185337). Required for acetylation of GJA1 in the developing cerebral cortex (By similarity). |
| Cellular Location | Cytoplasm. Nucleus [Isoform 2]: Cytoplasm. Nucleus |
| Tissue Location | Expressed in the cerebellum and spinal motor neurons. |
Provided below are standard protocols that you may find useful for product applications.
Background
Elongator complex protein 3 (ELP3) is a catalytic histone acetyltransferase subunit of the RNA polymerase II elongator complex, which is a component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Elongator may play a role in chromatin remodeling and is involved in acetylation of histones H3 and probably H4. It may also have a methyltransferase activity.
References
Han,Q., Acta Biochim. Biophys. Sin. (Shanghai) 39 (6), 453-461 (2007)
Kim,J.H., Proc. Natl. Acad. Sci. U.S.A. 99 (3), 1241-1246 (2002)
Ninomiya,Y., J. Biochem. 118 (2), 380-389 (1995)
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