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PHF12 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
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- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | Q96QT6 |
| Other Accession | Q5SPL2, NP_001028733.1, NP_065940.1 |
| Reactivity | Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 109698 Da |
| Antigen Region | 831-859 aa |
| Gene ID | 57649 |
|---|---|
| Other Names | PHD finger protein 12, PHD factor 1, Pf1, PHF12 (HGNC:20816) |
| Target/Specificity | This PHF12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 831-859 amino acids from the C-terminal region of human PHF12. |
| Dilution | WB~~1:1000 IHC-P~~1:50~100 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | PHF12 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PHF12 (HGNC:20816) |
|---|---|
| Function | Transcriptional repressor acting as key scaffolding subunit of SIN3 complexes which contributes to complex assembly by contacting each core subunit domain, stabilizes the complex and constitutes the substrate receptor by recruiting the H3 histone tail (PubMed:37137925). SIN3 complexes are composed of a SIN3 scaffold subunit, one catalytic core (HDAC1 or HDAC2) and 2 chromatin targeting modules (PubMed:11390640, PubMed:37137925). SIN3B complex represses transcription and counteracts the histone acetyltransferase activity of EP300 through the recognition H3K27ac marks by PHF12 and the activity of the histone deacetylase HDAC2 (PubMed:37137925). SIN3B complex is recruited downstream of the constitutively active genes transcriptional start sites through interaction with histones and mitigates histone acetylation and RNA polymerase II progression within transcribed regions contributing to the regulation of transcription (PubMed:21041482). May also repress transcription in a SIN3A- independent manner through recruitment of functional TLE5 complexes to DNA (PubMed:11390640). May also play a role in ribosomal biogenesis (By similarity). |
| Cellular Location | Nucleus. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.
References
Nousiainen, M., et al. Proc. Natl. Acad. Sci. U.S.A. 103(14):5391-5396(2006)
Nousiainen, M., et al. Proc. Natl. Acad. Sci. U.S.A. 103(14):5391-5396(2006)
Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004)
Yochum, G.S., et al. Mol. Cell. Biol. 22(22):7868-7876(2002)
Yochum, G.S., et al. Mol. Cell. Biol. 21(13):4110-4118(2001)
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