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OTOA Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - OTOA Antibody (N-term) AP11149a
    OTOA Antibody (N-term) (Cat. #AP11149a) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the OTOA antibody detected the OTOA protein (arrow).
    detail
  • IHC-P - OTOA Antibody (N-term) AP11149a
    OTOA Antibody (N-term) (Cat. #AP11149a)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of OTOA Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q7RTW8
Other Accession NP_733764.1, NP_001155155.1
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 128533 Da
Antigen Region 92-120 aa
Additional Information
Gene ID 146183
Other Names Otoancorin, OTOA
Target/Specificity This OTOA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 92-120 amino acids from the N-terminal region of human OTOA.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsOTOA Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name OTOA
Function May act as an adhesion molecule.
Cellular Location Apical cell membrane; Lipid-anchor, GPI-anchor; Extracellular side. Secreted, extracellular space, extracellular matrix. Note=At the interface between the apical surface of the epithelia and the overlying acellular gel of the tectorial and otoconial membranes.
Research Areas
Citations (0)
citation

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Background

The protein encoded by this gene is specifically expressed in the inner ear, and is located at the interface between the apical surface of the inner ear sensory epithelia and their overlying acellular gels. It is prposed that this protein is involved in the attachment of the inner ear acellular gels to the apical surface of the underlying nonsensory cells. Mutations in this gene are associated with autosomal recessive deafness type 22 (DFNB22). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

References

Shahin, H., et al. Eur. J. Hum. Genet. 18(4):407-413(2010)
Rose, J. Phd, et al. Mol. Med. (2010) In press :
Hofmann, O., et al. Proc. Natl. Acad. Sci. U.S.A. 105(51):20422-20427(2008)
Zwaenepoel, I., et al. Proc. Natl. Acad. Sci. U.S.A. 99(9):6240-6245(2002)

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$ 365.00
$ 140.00
Cat# AP11149a
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