|Application ||WB, IHC-P, FC, E|
|Other Accession||Q641Y5, Q9D906, Q5ZKY2, NP_006386.1|
|Predicted||Chicken, Mouse, Rat|
|Calculated MW||77960 Da|
|Antigen Region||540-569 aa|
|Other Names||Ubiquitin-like modifier-activating enzyme ATG7, ATG12-activating enzyme E1 ATG7, Autophagy-related protein 7, APG7-like, hAGP7, Ubiquitin-activating enzyme E1-like protein, ATG7, APG7L|
|Target/Specificity||This APG7L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 540-569 amino acids from human APG7L.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||APG7L Antibody(D555) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||E1-like activating enzyme involved in the 2 ubiquitin- like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation.|
|Cellular Location||Cytoplasm. Preautophagosomal structure. Note=Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme|
|Tissue Location||Widely expressed, especially in kidney, liver, lymph nodes and bone marrow.|
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Provided below are standard protocols that you may find useful for product applications.
This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes.
Metzger, S., et al. Hum. Genet. 128(4):453-459(2010)
Zhao, Y., et al. Nat. Cell Biol. 12(7):665-675(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Xue, L.Y., et al. Autophagy 6(2):248-255(2010)
Zhu, K., et al. Oncogene 29(3):451-462(2010)
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