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APG7L Antibody(D555)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP11191a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP11191a-ev20 | Ev20 size 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
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- Backgrounds
APG7L Antibody(D555) - Product info | |
| Application | WB, IHC, FC
|
| Primary Accession | O95352 |
| Other Accession | Q641Y5, Q9D906, Q5ZKY2, NP_006386.1 |
| Reactivity | Human |
| Predicted | Chicken, Mouse, Rat |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 77960 Da |
APG7L Antibody(D555) - Additional info | |
| Gene ID 10533 | |
| Other Names ATG7; APG7L; Ubiquitin-like modifier-activating enzyme ATG7; ATG12-activating enzyme E1 ATG7; Autophagy-related protein 7; Ubiquitin-activating enzyme E1-like protein | |
| Target/Specificity This APG7L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 547-576 amino acids from human APG7L. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:50~100 FC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions APG7L Antibody(D555) is for research use only and not for use in diagnostic or therapeutic procedures. | |
APG7L Antibody(D555) - Protein Information | |
| Name ATG7 | |
| Synonyms APG7L | |
| Function Functions as an E1 enzyme essential for multisubstrates such as ATG8-like proteins and ATG12. Forms intermediate conjugates with ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). PE-conjugation to ATG8-like proteins is essential for autophagy. Also acts as an E1 enzyme for ATG12 conjugation to ATG5 and ATG3 (By similarity) | |
| Cellular Location Cytoplasm (Probable). | |
| Tissue Location Widely expressed, especially in kidney, liver, lymph nodes and bone marrow | |
APG7L Antibody(D555) - Related products
AP11191a: APG7L Antibody(D555)
AP1813a: ATG7 Antibody (N-term)
AP1813b: ATG7 Antibody (Center)
AP1813d: ATG7 Antibody (C-term)
AP3365a: Phospho-ATG7-S95 Antibody
DC00041: Human ATG7 cDNA Clone
BP1813a: APG7L Antibody (N-term) Blocking Peptide
BP1813b: APG7L Antibody (Center) Blocking Peptide
BP1813c: APG7L Antibody (R509) Blocking Peptide
BP1813d: APG7L Antibody (C-term) Blocking Peptide
BP1813e: APG7L Antibody (S173) Blocking Peptide
APG7L Antibody(D555) - Application data
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hAPG7L-D555 (PEI 1:100)b (Cat. #AP11191a) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the APG7L antibody detected the APG7L protein (arrow).
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APG7L Antibody(D555) (Cat. #AP11191a)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of APG7L Antibody(D555) for immunohistochemistry. Clinical relevance has not been evaluated.
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APG7L Antibody (Cat. #AP11191a) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
APG7L Antibody(D555) - Research Areas
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BACKGROUND
This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes.
REFERENCES
Metzger, S., et al. Hum. Genet. 128(4):453-459(2010) Zhao, Y., et al. Nat. Cell Biol. 12(7):665-675(2010) Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) : Xue, L.Y., et al. Autophagy 6(2):248-255(2010) Zhu, K., et al. Oncogene 29(3):451-462(2010)
