|Application ||FC, WB, E|
|Calculated MW||134466 Da|
|Antigen Region||176-205 aa|
|Other Names||Leucine--tRNA ligase, cytoplasmic, Leucyl-tRNA synthetase, LeuRS, LARS, KIAA1352|
|Target/Specificity||This LARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 176-205 amino acids from the N-terminal region of human LARS.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||LARS Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the specific attachment of an amino acid to its cognate tRNA in a two step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Exhibits a post-transfer editing activity to hydrolyze mischarged tRNAs.|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a cytosolic leucine-tRNA synthetase, a member of the class I aminoacyl-tRNA synthetase family. The encoded enzyme catalyzes the ATP-dependent ligation of L-leucine to tRNA(Leu). It is found in the cytoplasm as part of a multisynthetase complex and interacts with the arginine tRNA synthetase through its C-terminal domain. Alternatively spliced transcript variants of this gene have been found; however, their full-length nature is not known.
Pang, Y.L., et al. Biochemistry 48(38):8958-8964(2009)
Seiradake, E., et al. J. Mol. Biol. 390(2):196-207(2009)
Shin, S.H., et al. Exp. Mol. Med. 40(2):229-236(2008)
Maeso, E., et al. Neuromuscul. Disord. 17(5):415-418(2007)
Lue, S.W., et al. Biochemistry 46(15):4466-4472(2007)
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