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FBXO18 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - FBXO18 Antibody (C-term) AP11216b
    FBXO18 Antibody (C-term) (Cat. #AP11216b) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the FBXO18 antibody detected the FBXO18 protein (arrow).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q8NFZ0
Other Accession NP_835363
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 117686 Da
Antigen Region 741-769 aa
Additional Information
Gene ID 84893
Other Names F-box only protein 18, F-box DNA helicase 1, FBXO18, FBH1, FBX18
Target/Specificity This FBXO18 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 741-769 amino acids from the C-terminal region of human FBXO18.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFBXO18 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FBXO18 (HGNC:13620)
Function 3'-5' DNA helicase and substrate-recognition component of the SCF(FBXO18) E3 ubiquitin ligase complex that plays a key role in response to stalled/damaged replication forks (PubMed:11956208, PubMed:23393192). Involved in genome maintenance by acting as an anti-recombinogenic helicase and preventing extensive strand exchange during homologous recombination: promotes RAD51 filament dissolution from stalled forks, thereby inhibiting homologous recombination and preventing excessive recombination (PubMed:17724085, PubMed:19736316). Also promotes cell death and DNA double-strand breakage in response to replication stress: together with MUS81, promotes the endonucleolytic DNA cleavage following prolonged replication stress via its helicase activity, possibly to eliminate cells with excessive replication stress (PubMed:23319600, PubMed:23361013). Plays a major role in remodeling of stalled DNA forks by catalyzing fork regression, in which the fork reverses and the two nascent DNA strands anneal (PubMed:25772361). In addition to the helicase activity, also acts as the substrate-recognition component of the SCF(FBXO18) E3 ubiquitin ligase complex, a complex that mediates ubiquitination of RAD51, leading to regulate RAD51 subcellular location (PubMed:25585578).
Cellular Location Nucleus. Chromosome. Note=Accumulates at sites of DNA damage or replication stress (PubMed:19736316, PubMed:23677613) PCNA is required for localization to DNA damage sites (PubMed:23677613). Localizes to the nucleoplasm in absence of DNA damage (PubMed:23677613).
Research Areas
Citations (0)

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Background

This gene encodes a member of the F-box protein family, members of which are characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into three classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbx class. It contains an F-box motif and seven conserved helicase motifs, and has both DNA-dependent ATPase and DNA unwinding activities. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene.

References

Turnbull, C., et al. Nat. Genet. 42(6):504-507(2010)
Fugger, K., et al. J. Cell Biol. 186(5):655-663(2009)
Chiolo, I., et al. Mol. Cell. Biol. 27(21):7439-7450(2007)
Grupe, A., et al. Am. J. Hum. Genet. 78(1):78-88(2006)
Deloukas, P., et al. Nature 429(6990):375-381(2004)

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Discontinued
Cat# AP11216b
Size:
(40 western blots)

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