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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   NCOR1 Antibody (N-term)   

NCOR1 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - NCOR1 Antibody (N-term) AP1121A
    The anti-NCOR1 Pab (Cat. #AP1121a) is used in Western blot to detect NCOR1 in mouse thymus tissue lysate.
  • IHC-P - NCOR1 Antibody (N-term) AP1121A
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
  • IF - NCOR1 Antibody (N-term) AP1121A
    Fluorescent confocal image of Hela cell stained with (MOUSE) Ncor1 Antibody(Cat#AP1121a). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with Ncor1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). Ncor1 immunoreactivity is localized to nucleus significantly.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession O75376
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 270210 Da
Antigen Region 16-47 aa
Additional Information
Gene ID 9611
Other Names Nuclear receptor corepressor 1, N-CoR, N-CoR1, NCOR1, KIAA1047
Target/Specificity This NCOR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-47 amino acids from the N-terminal region of human NCOR1.
Dilution WB~~1:1000
IHC-P~~1:50~100
IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNCOR1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NCOR1
Synonyms KIAA1047
Function Mediates transcriptional repression by certain nuclear receptors. Part of a complex which promotes histone deacetylation and the formation of repressive chromatin structures which may impede the access of basal transcription factors. Participates in the transcriptional repressor activity produced by BCL6.
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00624}
Research Areas
Citations (0)

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Background

NCOR1 mediates ligand-independent transcription repression of thyroid-hormone and retinoic-acid receptors by promoting chromatin condensation and preventing access of the transcription machinery. It is part of a complex which also includes histone deacetylases and transcriptional regulators similar to the yeast protein Sin3p. The gene is located between the Charcot-Marie-Tooth and Smith-Magenis syndrome critical regions on chromosome 17. An alternatively spliced transcript variant has been described, but its full length sequence has not been determined.

References

Tai, H.H., et al., Biochem. Biophys. Res. Commun. 308(1):170-176 (2003).
Ishizuka, T., et al., Mol. Cell. Biol. 23(15):5122-5131 (2003).
Loinder, K., et al., J. Steroid Biochem. Mol. Biol. 84(1):15-21 (2003).
Wang, S., et al., Oncogene 21(55):8388-8396 (2002).
Baek, S.H., et al., Cell 110(1):55-67 (2002).

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Cat# AP1121A
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(40 western blots)
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