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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   IGF2 Antibody (Center R54)   

IGF2 Antibody (Center R54)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • FC - IGF2 Antibody (Center R54) AP11220c
    Overlay histogram showing Hela cells stained with AP11220c (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP11220c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • WB - IGF2 Antibody (Center R54) AP11220c
    All lanes : Anti-IGF2 Antibody (Center R54) at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 20 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - IGF2 Antibody (Center R54) AP11220c
    Anti-IGF2 Antibody (Center R54) at 1:2000 dilution + mouse liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 20 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IHC-P - IGF2 Antibody (Center R54) AP11220c
    IGF2 Antibody (Center R54) (Cat. #AP11220c)immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IGF2 Antibody (Center R54) for immunohistochemistry. Clinical relevance has not been evaluated.
  • WB - IGF2 Antibody (Center R54) AP11220c
    IGF2 Antibody (Center R54) (Cat. #AP11220c) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the IGF2 antibody detected the IGF2 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P01344
Other Accession NP_000603
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 20140 Da
Antigen Region 39-68 aa
Additional Information
Gene ID 3481
Other Names Insulin-like growth factor II, IGF-II, Somatomedin-A, Insulin-like growth factor II, Insulin-like growth factor II Ala-25 Del, Preptin, IGF2
Target/Specificity This IGF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-68 amino acids from the Central region of human IGF2.
Dilution FC~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsIGF2 Antibody (Center R54) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IGF2
Function The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.
Cellular Location Secreted.
Research Areas
Citations (0)

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This gene encodes a member of the insulin family of polypeptide growth factors, which are involved in development and growth. It is an imprinted gene, expressed only from the paternal allele, and epigenetic changes at this locus are associated with Wilms tumour, Beckwith-Wiedemann syndrome, rhabdomyosarcoma, and Silver-Russell syndrome. A read-through INS-IGF2 gene exists, whose 5' region overlaps the INS gene and the 3' region overlaps this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.


Adkins, R.M., et al. Pediatr. Res. 68(5):429-434(2010)
Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) :
Li, J., et al. Mol. Biol. Rep. (2010) In press :
Hsieh, Y.Y., et al. Anticancer Res. 30(6):2203-2208(2010)
Turan, N., et al. PLoS Genet. 6 (7), E1001033 (2010) :

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$ 295.00
$ 99.00
Cat# AP11220c
(40 western blots)
Availability: In Stock
Bulk Size
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