DUOX2 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application
| FC, WB, E |
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Primary Accession | Q9NRD8 |
Other Accession | NP_054799 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 175364 Da |
Antigen Region | 513-542 aa |
Gene ID | 50506 |
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Other Names | Dual oxidase 2, 1111-, Large NOX 2, Long NOX 2, NADH/NADPH thyroid oxidase p138-tox, NADPH oxidase/peroxidase DUOX2, NADPH thyroid oxidase 2, Thyroid oxidase 2, p138 thyroid oxidase, DUOX2, LNOX2, THOX2 |
Target/Specificity | This DUOX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 513-542 amino acids from the Central region of human DUOX2. |
Dilution | WB~~1:1000 FC~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | DUOX2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | DUOX2 |
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Synonyms | LNOX2, THOX2 |
Function | Generates hydrogen peroxide which is required for the activity of thyroid peroxidase/TPO and lactoperoxidase/LPO. Plays a role in thyroid hormones synthesis and lactoperoxidase-mediated antimicrobial defense at the surface of mucosa. May have its own peroxidase activity through its N-terminal peroxidase-like domain. |
Cellular Location | Apical cell membrane; Multi-pass membrane protein. Cell junction. Note=Localizes to the apical membrane of epithelial cells. Localizes on internal membrane structures under resting conditions, translocates to the plasma membrane and cell-cell junctions upon challenge with enteric pathogens, such as Escherichia coli. |
Tissue Location | Expressed in colon, small intestine, duodenum and tracheal surface epithelial cells (at protein level). Expressed in thyrocytes. Also detected in kidney, liver, lung, pancreas, prostate, salivary glands, rectum and testis. |
Provided below are standard protocols that you may find useful for product applications.
Background
The protein encoded by this gene is a glycoprotein and a member of the NADPH oxidase family. The synthesis of thyroid hormone is catalyzed by a protein complex located at the apical membrane of thyroid follicular cells. This complex contains an iodide transporter, thyroperoxidase, and a peroxide generating system that includes this encoded protein and DUOX1. This protein is known as dual oxidase because it has both a peroxidase homology domain and a gp91phox domain.
References
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Salmeen, A., et al. Oncogene 29(31):4473-4484(2010)
Linderholm, A.L., et al. Am. J. Physiol. Lung Cell Mol. Physiol. 299 (2), L215-L221 (2010) :
Roberts, K.E., et al. Gastroenterology 139(1):130-139(2010)
Hill, T. III, et al. Biochem. Biophys. Res. Commun. 395(2):270-274(2010)
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