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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   DPP3 Antibody (C-term)   

DPP3 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - DPP3 Antibody (C-term) AP11232B
    DPP3 Antibody (C-term) (Cat. #AP11232b) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the DPP3 antibody detected the DPP3 protein (arrow).
  • IHC-P - DPP3 Antibody (C-term) AP11232B
    DPP3 Antibody (C-term) (Cat. #AP11232b)immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DPP3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - DPP3 Antibody (C-term) AP11232B
    DPP3 Antibody (C-term) (Cat. #AP11232b) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • IF - DPP3 Antibody (C-term) AP11232B
    Confocal immunofluorescent analysis of DPP3 Antibody (C-term)(Cat#AP11232b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, IF, E
Primary Accession Q9NY33
Other Accession Q99KK7, NP_005691
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 82589 Da
Antigen Region 583-612 aa
Additional Information
Gene ID 10072
Other Names Dipeptidyl peptidase 3, Dipeptidyl aminopeptidase III, Dipeptidyl arylamidase III, Dipeptidyl peptidase III, DPP III, Enkephalinase B, DPP3
Target/Specificity This DPP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 583-612 amino acids from the C-terminal region of human DPP3.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDPP3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DPP3
Function Cleaves and degrades of the opioid peptide enkephalin. Also cleaves Arg-Arg-beta-naphthylamide.
Cellular Location Cytoplasm.
Research Areas
Citations (0)

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Background

This gene encodes a protein that is a member of the M49 family of metallopeptidases. This cytoplasmic protein binds a single zinc ion with its zinc-binding motif (HELLGH) and has post-proline dipeptidyl aminopeptidase activity, cleaving Xaa-Pro dipeptides from the N-termini of proteins. Increased activity of this protein is associated with endometrial and ovarian cancers. Alternate transcriptional splice variants have been characterized.

References

Shukla, A.A., et al. FEBS J. 277(8):1861-1875(2010)
Salopek-Sondi, B., et al. Biol. Chem. 389(2):163-167(2008)
Gevaert, K., et al. Proteomics 5(14):3589-3599(2005)
Abramic, M., et al. Int. J. Biochem. Cell Biol. 36(3):434-446(2004)
Hillman, R.T., et al. Genome Biol. 5 (2), R8 (2004) :

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$ 295.00
$ 99.00
Cat# AP11232B
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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