TSSK4 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| IF, IHC-P, WB, E |
---|---|
Primary Accession | Q6SA08 |
Other Accession | Q9D411, NP_777604.2 |
Reactivity | Human |
Predicted | Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 37454 Da |
Antigen Region | 226-254 aa |
Gene ID | 283629 |
---|---|
Other Names | Testis-specific serine/threonine-protein kinase 4, TSK-4, TSSK-4, Testis-specific kinase 4, Serine/threonine-protein kinase 22E, TSSK4, C14orf20, STK22E, TSSK5 |
Target/Specificity | This TSSK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 226-254 amino acids from the C-terminal region of human TSSK4. |
Dilution | IF~~1:10~50 WB~~1:1000 IHC-P~~1:50~100 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | TSSK4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | TSSK4 (HGNC:19825) |
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Function | Serine/threonine kinase which is involved in male germ cell development and in mature sperm function (By similarity). May be involved in the Cre/Creb signaling pathway (By similarity). Phosphorylates CREB1 on 'Ser-133' in vitro and can stimulate Cre/Creb pathway in cells (PubMed:15964553). Phosphorylates CREM on 'Ser-116' in vitro (By similarity). Phosphorylates ODF2 on 'Ser-95' (By similarity). |
Cellular Location | Cytoplasmic vesicle, secretory vesicle, acrosome {ECO:0000250|UniProtKB:Q9D411}. Cell projection, cilium, flagellum {ECO:0000250|UniProtKB:Q9D411}. Note=In spermatozoa, present in the sperm head and in the flagellum. {ECO:0000250|UniProtKB:Q9D411} |
Tissue Location | Expressed only in the testis. |
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes a member of the testis-specific serine/threonine kinase family. The encoded protein is thought to be involved in spermatogenesis via stimulation of the CREB/CRE responsive pathway through phosphorylation of the cAMP responsive element binding protein transcription factor. Alternative splicing results in multiple transcript variants.
References
Aston, K.I., et al. Hum. Reprod. 25(6):1383-1397(2010)
Su, D., et al. J. Androl. 29(4):374-378(2008)
Zeng, M., et al. BMB Rep 41(4):300-304(2008)
Matsuoka, S., et al. Science 316(5828):1160-1166(2007)
Chen, X., et al. Biochem. Biophys. Res. Commun. 333(3):742-749(2005)
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