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TSSK4 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - TSSK4 Antibody (C-term) AP11247b
    Western blot analysis of TSSK4 (arrow) using rabbit polyclonal TSSK4 Antibody (C-term) (Cat. #AP11247b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TSSK4 gene.
  • IHC-P - TSSK4 Antibody (C-term) AP11247b
    TSSK4 Antibody (C-term) (Cat. #AP11247b)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TSSK4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • IF - TSSK4 Antibody (C-term) AP11247b
    Confocal immunofluorescent analysis of TSSK4 Antibody (C-term)(Cat#AP11247b) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q6SA08
Other Accession Q9D411, NP_777604.2
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 37454 Da
Antigen Region 226-254 aa
Additional Information
Gene ID 283629
Other Names Testis-specific serine/threonine-protein kinase 4, TSK-4, TSSK-4, Testis-specific kinase 4, Serine/threonine-protein kinase 22E, TSSK4, C14orf20, STK22E, TSSK5
Target/Specificity This TSSK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 226-254 amino acids from the C-terminal region of human TSSK4.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTSSK4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TSSK4
Synonyms C14orf20, STK22E, TSSK5
Function May be involved in a signaling pathway during male germ cell development or mature sperm function (By similarity). Phosphorylates CREB1 on Ser-133 and stimulates downstream signaling.
Tissue Location Expressed only in the testis.
Citations (0)

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This gene encodes a member of the testis-specific serine/threonine kinase family. The encoded protein is thought to be involved in spermatogenesis via stimulation of the CREB/CRE responsive pathway through phosphorylation of the cAMP responsive element binding protein transcription factor. Alternative splicing results in multiple transcript variants.


Aston, K.I., et al. Hum. Reprod. 25(6):1383-1397(2010)
Su, D., et al. J. Androl. 29(4):374-378(2008)
Zeng, M., et al. BMB Rep 41(4):300-304(2008)
Matsuoka, S., et al. Science 316(5828):1160-1166(2007)
Chen, X., et al. Biochem. Biophys. Res. Commun. 333(3):742-749(2005)

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Cat# AP11247b
(40 western blots)
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