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KRT25 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, FC, E |
|---|---|
| Primary Accession | Q7Z3Z0 |
| Other Accession | NP_853512.1 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 49318 Da |
| Antigen Region | 380-408 aa |
| Gene ID | 147183 |
|---|---|
| Other Names | Keratin, type I cytoskeletal 25, Cytokeratin-25, CK-25, Keratin-25, K25, Keratin-25A, K25A, Type I inner root sheath-specific keratin-K25irs1, KRT25 (HGNC:30839) |
| Target/Specificity | This KRT25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 380-408 amino acids from the C-terminal region of human KRT25. |
| Dilution | WB~~1:1000 FC~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | KRT25 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | KRT25 (HGNC:30839) |
|---|---|
| Function | Essential for the proper assembly of type I and type II keratin protein complexes and formation of keratin intermediate filaments in the inner root sheath (irs) (By similarity). Plays a role in the cytoskeleton organization (PubMed:26902920). |
| Cellular Location | Cytoplasm {ECO:0000250|UniProtKB:Q8VCW2}. |
| Tissue Location | Strongly expressed in skin and scalp, and weak expression observed in thymus and tongue. In the hair follicle, expressed in Henle layer, Huxley layer and in the inner root sheath cuticle of the hair follicle. Expression extends from the bulb region up to the point of differentiation into the three layers. Also present in the medulla of beard hair (at protein level) |
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes a member of the type I (acidic) keratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. The type I keratin genes are clustered in a region of chromosome 17q12-q21.
References
Rose, J. Phd, et al. Mol. Med. (2010) In press :
Schweizer, J., et al. J. Cell Biol. 174(2):169-174(2006)
Rogers, M.A., et al. Differentiation 72 (9-10), 527-540 (2004) :
Hesse, M., et al. Eur. J. Cell Biol. 83(1):19-26(2004)
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