|Application ||WB, E|
|Calculated MW||38244 Da|
|Antigen Region||83-112 aa|
|Other Names||Putative methyltransferase NSUN3, 211-, NOL1/NOP2/Sun domain family member 3, NSUN3|
|Target/Specificity||This NSUN3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 83-112 amino acids from the N-terminal region of human NSUN3.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||NSUN3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Mitochondrial tRNA methyltransferase that mediates methylation of cytosine to 5-methylcytosine (m5C) at position 34 of mt-tRNA(Met) (PubMed:27497299, PubMed:27214402, PubMed:27356879). mt-tRNA(Met) methylation at cytosine(34) takes place at the wobble position of the anticodon and initiates the formation of 5-formylcytosine (f(5)c) at this position (PubMed:27497299, PubMed:27214402, PubMed:27356879). mt-tRNA(Met) containing the f(5)c modification at the wobble position enables recognition of the AUA codon in addition to the AUG codon, expanding codon recognition in mitochondrial translation (PubMed:27497299, PubMed:27356879).|
|Cellular Location||Mitochondrion matrix|
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Provided below are standard protocols that you may find useful for product applications.
May have S-adenosyl-L-methionine-dependent methyl-transferase activity (Potential).
Trynka, G., et al. Gut 58(8):1078-1083(2009)
Lamesch, P., et al. Genomics 89(3):307-315(2007)
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