- CITATIONS: 1
|Application ||WB, IHC-P, IF, E|
|Other Accession||Q567B1, Q5F3A6, NP_061036.3|
|Calculated MW||38322 Da|
|Antigen Region||319-347 aa|
|Other Names||Ubiquitin thioesterase OTU1, DUBA-8, HIV-1-induced protease 7, HIN-7, HsHIN7, OTU domain-containing protein 2, YOD1, DUBA8, HIN7, OTUD2|
|Target/Specificity||This YOD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 319-347 amino acids from the C-terminal region of human YOD1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||YOD1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||DUBA8, HIN7, OTUD2|
|Function||Hydrolase that can remove conjugated ubiquitin from proteins and participates in endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal proteins. May act by triming the ubiquitin chain on the associated substrate to facilitate their threading through the VCP/p97 pore. Ubiquitin moieties on substrates may present a steric impediment to the threading process when the substrate is transferred to the VCP pore and threaded through VCP's axial channel. Mediates deubiquitination of 'Lys-27'-, 'Lys-29'- and 'Lys-33'-linked polyubiquitin chains. Also able to hydrolyze 'Lys-11'-linked ubiquitin chains. Cleaves both polyubiquitin and di-ubiquitin. May play a role in macroautophagy, regulating for instance the clearance of damaged lysosomes. May recruit PLAA, UBXN6 and VCP to damaged lysosome membranes decorated with K48-linked ubiquitin chains and remove these chains allowing autophagosome formation (PubMed:27753622).|
|Cellular Location||Cytoplasm. Note=Recruited to damaged lysosomes decorated with K48-linked ubiquitin chains.|
Provided below are standard protocols that you may find useful for product applications.
Deubiquitinating enzymes (DUBs; see MIM 603478) are proteases that specifically cleave ubiquitin (MIM 191339) linkages, negating the action of ubiquitin ligases. DUBA8 belongs to a DUB subfamily characterized by an ovarian tumor (OTU) domain.[supplied by OMIM].
Ernst, R., et al. Mol. Cell 36(1):28-38(2009)
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