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ARL8 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ARL8 Antibody (C-term) AP11320b
    ARL8 Antibody (C-term) (Cat. #AP11320b) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the ARL8 antibody detected the ARL8 protein (arrow).
    detail
  • IHC-P - ARL8 Antibody (C-term) AP11320b
    ARL8 Antibody (C-term) (Cat. #AP11320b)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ARL8 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - ARL8 Antibody (C-term) AP11320b
    ARL8 Antibody (C-term) (Cat. #AP11320b) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, WB, IHC-P, E
Primary Accession Q96KC2
Other Accession Q9D4P0, NP_848930.1
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 20375 Da
Antigen Region 122-154 aa
Additional Information
Gene ID 221079
Other Names ADP-ribosylation factor-like protein 5B, ADP-ribosylation factor-like protein 8, ARL5B, ARL8
Target/Specificity This ARL8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 122-154 amino acids from the C-terminal region of human ARL8.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsARL8 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ARL5B
Synonyms ARL8
Function Binds and exchanges GTP and GDP.
Research Areas
Citations (0)
citation

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Background

ARL5B (ARL8) belongs to a family of proteins that are structurally similar to ADP-ribosylation factors (ARFs; see MIM 103180). ARLs and ARFs are part of the RAS superfamily of regulatory GTPases.

References

Grupe, A., et al. Am. J. Hum. Genet. 78(1):78-88(2006)
Barrios-Rodiles, M., et al. Science 307(5715):1621-1625(2005)
Sebald, E., et al. Gene 311, 147-151 (2003) :

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$ 365.00
$ 140.00
Cat# AP11320b
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