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BIRC5 Antibody (N-term)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP11321a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP11321a-ev20 | 20 ug 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
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- Citiations : 0
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- Backgrounds
BIRC5 Antibody (N-term) - Product info | |
| Application | WB, IHC, IF
|
| Primary Accession | O15392 |
| Other Accession | Q9GLN5, NP_001012271.1, NP_001159.2 |
| Reactivity | Human |
| Predicted | Pig |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 16389 Da |
BIRC5 Antibody (N-term) - Additional info | |
| Gene ID 332 | |
| Other Names BIRC5; API4; IAP4; Baculoviral IAP repeat-containing protein 5; Apoptosis inhibitor 4; Apoptosis inhibitor survivin | |
| Target/Specificity This BIRC5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7-39 amino acids from the N-terminal region of human BIRC5. | |
| Dilution WB~~1:50~100WB~~1:1000 IHC~~1:50~100 IF~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions BIRC5 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
BIRC5 Antibody (N-term) - Protein Information | |
| Name BIRC5 | |
| Synonyms API4, IAP4 | |
| Function Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis. Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis. Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May counteract a default induction of apoptosis in G2/M phase. The acetylated form represses STAT3 transactivation of target gene promoters. May play a role in neoplasia. Inhibitor of CASP3 and CASP7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform | |
| Cellular Location Cytoplasm. Nucleus. Chromosome. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore. Midbody. Note=Localizes on chromosome arms and inner centromeres from prophase through metaphase Localizes to kinetochores in metaphase, distributes to the midzone microtubules in anaphase and at telophase, localizes exclusively to the midbody. Colocalizes with AURKB at mitotic chromosomes Acetylation at Lys-129 directs its localization to the nucleus by enhancing homodimerization and thereby inhibiting XPO1/CRM1- mediated nuclear export | |
| Tissue Location Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines | |
BIRC5 Antibody (N-term) - Related products
AP11321a: BIRC5 Antibody (N-term)
AP11677c: BIRC5 Antibody (Center)
RI10598: BIRC5 predesign siRNA
DC02466: Human BIRC5 cDNA Clone
LY12234a: BIRC5 Over-expression Lysate
BIRC5 Antibody (N-term) - Application data
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BIRC5 Antibody (N-term) (Cat. #AP11321a) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the BIRC5 antibody detected the BIRC5 protein (arrow).
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Western blot analysis of BIRC5 (arrow) using rabbit polyclonal BIRC5 Antibody (N-term) (Cat. #AP11321a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the BIRC5 gene.
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BIRC5 Antibody (N-term) (Cat. #AP11321a)immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of BIRC5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Confocal immunofluorescent analysis of BIRC5 Antibody (N-term)(Cat#AP11321a) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
BIRC5 Antibody (N-term) - Research Areas
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BACKGROUND
This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors yet low in adult tissues. Antisense transcripts are involved in the regulation of this gene's expression. At least four transcript variants encoding distinct isoforms have been found for this gene, but the full-length natures of only three of them have been determined.
REFERENCES
Nabilsi, N.H., et al. J. Endocrinol. 207(2):237-243(2010) Valenzuela, M., et al. J. Infect. Dis. 202(7):1021-1030(2010) Yoon, S., et al. FEBS Lett. 584(18):4048-4052(2010) Ezponda, T., et al. Clin. Cancer Res. 16(16):4113-4125(2010) Sawai, K., et al. Oncol. Res. 18 (11-12), 541-547 (2010) :
