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PTCHD1 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PTCHD1 Antibody (Center) AP11396c
    PTCHD1 Antibody (Center) (Cat. #AP11396c) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the PTCHD1 antibody detected the PTCHD1 protein (arrow).
  • IHC-P - PTCHD1 Antibody (Center) AP11396c
    PTCHD1 Antibody (Center) (Cat. #AP11396c)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PTCHD1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q96NR3
Other Accession Q14B62, NP_775766.2
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 101341 Da
Antigen Region 611-639 aa
Additional Information
Gene ID 139411
Other Names Patched domain-containing protein 1, PTCHD1
Target/Specificity This PTCHD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 611-639 amino acids from the Central region of human PTCHD1.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPTCHD1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PTCHD1 (HGNC:26392)
Function Required for the development and function of the thalamic reticular nucleus (TRN), a part of the thalamus that is critical for thalamocortical transmission, generation of sleep rhythms, sensorimotor processing and attention.
Cellular Location Cell membrane; Multi-pass membrane protein
Tissue Location Widely expressed, including in various regions of the brain with highest expression in the gray and white cerebellum, followed by the cerebellar vermis and the pituitary gland.
Research Areas
Citations (0)

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References

Pinto, D., et al. Nature (2010) In press :

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Cat# AP11396c
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(40 western blots)
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