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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   SP1 Antibody (C-term P692)   

SP1 Antibody (C-term P692)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - SP1 Antibody (C-term P692) AP11451b
    Anti-SP1 Antibody (C-term P692) at 1:500 dilution + Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 81 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - SP1 Antibody (C-term P692) AP11451b
    SP1 Antibody (C-term P692) (Cat. #AP11451b) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the SP1 antibody detected the SP1 protein (arrow).
  • IHC-P - SP1 Antibody (C-term P692) AP11451b
    SP1 Antibody (C-term P692) (Cat. #AP11451b)immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SP1 Antibody (C-term P692) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - SP1 Antibody (C-term P692) AP11451b
    SP1 Antibody (C-term P692) (Cat. #AP11451b) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • IF - SP1 Antibody (C-term P692) AP11451b
    Confocal immunofluorescent analysis of SP1 Antibody (C-term P692)(Cat#AP11451b) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, IF, E
Primary Accession P08047
Other Accession Q01714, O89090, NP_612482.2
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 80693 Da
Antigen Region 677-707 aa
Additional Information
Gene ID 6667
Other Names Transcription factor Sp1, SP1, TSFP1
Target/Specificity This SP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 677-707 amino acids from the C-terminal region of human SP1.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSP1 Antibody (C-term P692) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SP1
Synonyms TSFP1
Function Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR- alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression. In complex with ATF7IP, maintains telomerase activity in cancer cells by inducing TERT and TERC gene expression. Isoform 3 is a stronger activator of transcription than isoform 1. Positively regulates the transcription of the core clock component ARNTL/BMAL1.
Cellular Location Nucleus. Cytoplasm. Note=Nuclear location is governed by glycosylated/phosphorylated states. Insulin promotes nuclear location, while glucagon favors cytoplasmic location
Tissue Location Up-regulated in adenocarcinomas of the stomach (at protein level). Isoform 3 is ubiquitously expressed at low levels.
Research Areas
Citations ( 0 )

Background

Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression.

References

Pan, Q., et al. Biochem. Biophys. Res. Commun. 401(2):306-312(2010)
Mucha, M., et al. J. Neurosci. 30(40):13235-13245(2010)
Imanishi, M., et al. Biochem. Biophys. Res. Commun. 400(4):625-630(2010)
Jutooru, I., et al. J. Biol. Chem. 285(33):25332-25344(2010)
Logotheti, S., et al. FEBS J. 277(14):3014-3027(2010)

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$ 295.00
$ 99.00
Cat# AP11451b
Size:
Quantity:
(40 western blots)
Availability: In Stock
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