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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   RSBN1 Antibody (N-term)   

RSBN1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IHC-P - RSBN1 Antibody (N-term) AP11537a
    AP11537a staining RSBN1 in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • IHC-P - RSBN1 Antibody (N-term) AP11537a
    AP11537a staining RSBN1 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • WB - RSBN1 Antibody (N-term) AP11537a
    All lanes : Anti-RSBN1 Antibody (N-term) at 1:2000 dilution Lane 1: human liver lysate Lane 2: MCF-7 whole cell lysate Lane 3: HL-60 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 90 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - RSBN1 Antibody (N-term) AP11537a
    All lanes : Anti-RSBN1 Antibody (N-term) at 1:2000 dilution Lane 1: CCRF-CEM whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: human liver lysate Lane 4: MCF-7 whole cell lysate Lane 5: HL-60 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 90 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - RSBN1 Antibody (N-term) AP11537a
    RSBN1 Antibody (N-term) (Cat. #AP11537a) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the RSBN1 antibody detected the RSBN1 protein (arrow).
  • IHC-P - RSBN1 Antibody (N-term) AP11537a
    RSBN1 Antibody (N-term) (Cat. #AP11537a)immunohistochemistry analysis in formalin fixed and paraffin embedded human plecenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RSBN1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - RSBN1 Antibody (N-term) AP11537a
    RSBN1 Antibody (N-term) (Cat. #AP11537a) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, FC, E
Primary Accession Q5VWQ0
Other Accession NP_060834.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 90072 Da
Antigen Region 51-78 aa
Additional Information
Gene ID 54665
Other Names Round spermatid basic protein 1, RSBN1
Target/Specificity This RSBN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-78 amino acids from the N-terminal region of human RSBN1.
Dilution IHC-P~~1:10~50
WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRSBN1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RSBN1
Cellular Location Nucleus.
Research Areas
Citations (0)

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References

Rose, J. Phd, et al. Mol. Med. (2010) In press :
Prahalad, S., et al. Arthritis Rheum. 60(7):2124-2130(2009)
Cooper, J.D., et al. Nat. Genet. 40(12):1399-1401(2008)
Raychaudhuri, S., et al. Nat. Genet. 40(10):1216-1223(2008)
Smyth, D.J., et al. Diabetes 57(6):1730-1737(2008)

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$ 295.00
$ 99.00
Cat# AP11537a
Size:
Quantity:
(40 western blots)
Availability: In Stock
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