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PIK3R5 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PIK3R5 Antibody (C-term) AP11544b
    PIK3R5 Antibody (C-term) (Cat. #AP11544b) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the PIK3R5 antibody detected the PIK3R5 protein (arrow).
    detail
  • IHC-P - PIK3R5 Antibody (C-term) AP11544b
    PIK3R5 Antibody (C-term) (Cat. #AP11544b)immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PIK3R5 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - PIK3R5 Antibody (C-term) AP11544b
    PIK3R5 Antibody (C-term) (Cat. #AP11544b) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession Q8WYR1
Other Accession NP_001136105.1, NP_055123.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 97348 Da
Antigen Region 763-792 aa
Additional Information
Gene ID 23533
Other Names Phosphoinositide 3-kinase regulatory subunit 5, PI3-kinase regulatory subunit 5, PI3-kinase p101 subunit, Phosphatidylinositol 4, 5-bisphosphate 3-kinase regulatory subunit, PtdIns-3-kinase regulatory subunit, Protein FOAP-2, PtdIns-3-kinase p101, p101-PI3K, PIK3R5
Target/Specificity This PIK3R5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 763-792 amino acids from the C-terminal region of human PIK3R5.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPIK3R5 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PIK3R5
Function Regulatory subunit of the PI3K gamma complex. Required for recruitment of the catalytic subunit to the plasma membrane via interaction with beta-gamma G protein dimers. Required for G protein- mediated activation of PIK3CG (By similarity).
Cellular Location Nucleus {ECO:0000250|UniProtKB:O02696}. Cytoplasm {ECO:0000250|UniProtKB:O02696}. Cell membrane {ECO:0000250|UniProtKB:O02696}; Peripheral membrane protein {ECO:0000250|UniProtKB:O02696}. Note=Predominantly localized in the nucleus in absence of PIK3CG/p120. Colocalizes with PIK3CG/p120 in the cytoplasm. Translocated to the plasma membrane in a beta-gamma G protein-dependent manner. {ECO:0000250|UniProtKB:O02696}
Tissue Location Ubiquitously expressed with high expression in fetal brain compared to adult brain. Abundant expression is observed in cerebellum, cerebral cortex, cerebral meninges, and vermis cerebelli
Research Areas
Citations (0)
citation

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Background

Receptor-regulated class I phosphoinositide 3-kinases (PI3Ks) phosphorylate the membrane lipid phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) to PtdIns(3,4,5)P3, which in turn recruits and activates cytosolic effectors involved in proliferation, survival, or chemotaxis. PIK3R5 is a PI3K regulatory subunit (Brock et al., 2003 [PubMed 12507995]).

References

Rose, J. Phd, et al. Mol. Med. (2010) In press :
Segat, L., et al. Vaccine 28(10):2201-2206(2010)
Yerges, L.M., et al. J. Bone Miner. Res. 24(12):2039-2049(2009)
Johnson, C., et al. Oncogene 26(49):7049-7057(2007)
Suire, S., et al. Curr. Biol. 15(6):566-570(2005)

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$ 182.50
$ 70.00
Cat# AP11544b
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