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RRAS Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - RRAS Antibody (Center) AP11548c
    RRAS Antibody (Center) (Cat. #AP11548c) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the RRAS antibody detected the RRAS protein (arrow).
    detail
  • WB - RRAS Antibody (Center) AP11548c
    Anti-RRAS Antibody (Center) at 1:1000 dilution + HT-29 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 23 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P10301
Other Accession P10833, NP_006261.1
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 23480 Da
Antigen Region 91-123 aa
Additional Information
Gene ID 6237
Other Names Ras-related protein R-Ras, p23, RRAS
Target/Specificity This RRAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 91-123 amino acids from the Central region of human RRAS.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRRAS Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RRAS
Function Regulates the organization of the actin cytoskeleton (PubMed:16537651, PubMed:18270267). With OSPBL3, modulates integrin beta-1 (ITGB1) activity (PubMed:18270267).
Cellular Location Cell membrane; Lipid-anchor; Cytoplasmic side. Note=Inner surface of plasma membrane possibly with attachment requiring acylation of the C- terminal cysteine (By similarity with RAS)
Research Areas
Citations (0)
citation

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Background

RRAS regulates the organization of the actin cytoskeleton.

References

Sakurai, A., et al. Mol. Cell. Biol. 30(12):3086-3098(2010)
Tong, Y., et al. J. Biol. Chem. 284(51):35962-35972(2009)
Inuzuka, T., et al. Biochem. Biophys. Res. Commun. 379(2):510-513(2009)
Xu, L., et al. J. Biol. Chem. 284(5):2706-2718(2009)
Hodkinson, P.S., et al. J. Biol. Chem. 282(39):28991-29001(2007)

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$ 182.50
$ 70.00
Cat# AP11548c
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