|Application ||WB, IHC-P, FC, E|
|Other Accession||Q8C2Q3, Q5EA36, NP_006319.1|
|Calculated MW||69492 Da|
|Antigen Region||563-594 aa|
|Other Names||RNA-binding protein 14, Paraspeckle protein 2, PSP2, RNA-binding motif protein 14, RRM-containing coactivator activator/modulator, Synaptotagmin-interacting protein, SYT-interacting protein, RBM14, SIP|
|Target/Specificity||This RBM14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 563-594 amino acids from the C-terminal region of human RBM14.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||RBM14 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Isoform 1 may function as a nuclear receptor coactivator, enhancing transcription through other coactivators such as NCOA6 and CITED1. Isoform 2, functions as a transcriptional repressor, modulating transcriptional activities of coactivators including isoform 1, NCOA6 and CITED1 (PubMed:11443112). Regulates centriole biogenesis by suppressing the formation of aberrant centriolar protein complexes in the cytoplasm and thus preserving mitotic spindle integrity. Prevents the formation of the STIL-CENPJ complex (which can induce the formation of aberrant centriolar protein complexes) by interfering with the interaction of STIL with CENPJ (PubMed:25385835).|
|Cellular Location||Nucleus. Nucleus, nucleolus. Cytoplasm. Note=In punctate subnuclear structures often located adjacent to splicing speckles, called paraspeckles (PubMed:11790299). Cytoplasmic localization is crucial for its function in suppressing the formation of aberrant centriolar protein complexes (PubMed:25385835)|
|Tissue Location||Expressed in all tissues tested, including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood lymphocytes|
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Provided below are standard protocols that you may find useful for product applications.
Isoform 1 may function as a nuclear receptor coactivator, enhancing transcription through other coactivators such as NCOA6 and CITED1. Isoform 2, functions as a transcriptional repressor, modulating transcriptional activities of coactivators including isoform 1, NCOA6 and CITED1.
Li, X., et al. J. Cell. Biochem. 108(2):378-387(2009)
Brooks, Y.S., et al. J. Biol. Chem. 284(27):18033-18046(2009)
Kang, Y.K., et al. Cancer Res. 68(19):7887-7896(2008)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Matsuoka, S., et al. Science 316(5828):1160-1166(2007)
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