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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   MITF Antibody (N-term)   

MITF Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - MITF Antibody (N-term) AP11559a
    Anti-MITF Antibody (N-term)at 1:2000 dilution + A375 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 59 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - MITF Antibody (N-term) AP11559a
    MITF Antibody (N-term) (Cat. #AP11559a) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the MITF antibody detected the MITF protein (arrow).
  • IF - MITF Antibody (N-term) AP11559a
    Confocal immunofluorescent analysis of MITF Antibody (N-term)(Cat#AP11559a) with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
  • FC - MITF Antibody (N-term) AP11559a
    MITF Antibody (N-term) (Cat. #AP11559a) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O75030
Other Accession Q08874, NP_937802.1, NP_001171896.1
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 58795 Da
Antigen Region 1-28 aa
Additional Information
Gene ID 4286
Other Names Microphthalmia-associated transcription factor, Class E basic helix-loop-helix protein 32, bHLHe32, MITF, BHLHE32
Target/Specificity This MITF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-28 amino acids from the N-terminal region of human MITF.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMITF Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms BHLHE32
Function Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to symmetrical DNA sequences (E-boxes) (5'- CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest- derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.
Cellular Location Nucleus.
Tissue Location Isoform M is exclusively expressed in melanocytes and melanoma cells. Isoform A and isoform H are widely expressed in many cell types including melanocytes and retinal pigment epithelium (RPE). Isoform C is expressed in many cell types including RPE but not in melanocyte-lineage cells. Isoform Mdel is widely expressed in melanocytes, melanoma cell lines and tissues, but almost undetectable in non-melanoma cell lines
Research Areas
Citations (0)

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This gene encodes a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retinal pigment epithelium and is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.


Wang, Y., et al. BMC Med 8, 14 (2010) : Shiohara, M., et al. Int J Lab Hematol 31(2):215-226(2009) Hershey, C.L., et al. Gene 347(1):73-82(2005) Miller, A.J., et al. J. Biol. Chem. 280(1):146-155(2005) Shibahara, S., et al. J. Investig. Dermatol. Symp. Proc. 6(1):99-104(2001)

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$ 295.00
$ 99.00
Cat# AP11559a
(40 western blots)
Availability: In Stock
Bulk Size
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