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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   OPN-a/b Antibody (N-term)   

OPN-a/b Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - OPN-a/b Antibody (N-term) AP11567a
    All lanes : Anti-OPN-a/b Antibody (N-term) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HL-60 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: MOLT-4 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 35 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IF - OPN-a/b Antibody (N-term) AP11567a
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized MCF-7 (human breast cancer cell line) cells labeling Pdx1 with AP11567a at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on MCF-7 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
  • WB - OPN-a/b Antibody (N-term) AP11567a
    Anti-OPN-a/b Antibody (N-term) at 1:2000 dilution + Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 35 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - OPN-a/b Antibody (N-term) AP11567a
    All lanes : Anti-OPN-a/b Antibody (N-term) at 1:1000 dilution Lane 1: human liver lysates Lane 2: MDA-MB-453 whole cell lysates Lane 3: MOLT-4 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 35 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - OPN-a/b Antibody (N-term) AP11567a
    Western blot analysis of OPN-a/b (arrow) using rabbit polyclonal OPN-a/b Antibody (N-term) (Cat. #AP11567a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the OPN-a/b gene.
  • IHC-P - OPN-a/b Antibody (N-term) AP11567a
    OPN-a/b Antibody (N-term) (Cat. #AP11567a)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of OPN-a/b Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • SPECIFICATION
  • CITATIONS: 4
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, IHC-P, E
Primary Accession P10451
Other Accession NP_000573.1, NP_001035147.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 35423 Da
Antigen Region 14-40 aa
Additional Information
Gene ID 6696
Other Names Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, SPP-1, Urinary stone protein, Uropontin, SPP1, BNSP, OPN
Target/Specificity This OPN-a/b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14-40 amino acids from the N-terminal region of human OPN-a/b.
Dilution WB~~1:1000
IF~~1:25
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsOPN-a/b Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SPP1
Synonyms BNSP, OPN
Function Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Cellular Location Secreted.
Tissue Location Bone. Found in plasma.
Research Areas
Citations ( 0 )

Background

Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity (By similarity).

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Weber, G.F., et al. Br. J. Cancer 103(6):861-869(2010)
Chen, R.X., et al. J. Gastroenterol. Hepatol. 25(8):1435-1442(2010)
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :
Johnatty, S.E., et al. PLoS Genet. 6 (7), E1001016 (2010) :

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$ 295.00
$ 99.00
Cat# AP11567a
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(40 western blots)
Availability: In Stock
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